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Fig. 3. Modulating retinoid signaling affects the expression of XCAD3 [cdx4] but not XCAD1 [cdx2] or XCAD2 [cdx1] . (A-C,F-H,K-M) Embryos were treated with the indicated compound or ethanol solvent controls from the early blastula stage (stage 7) until harvesting when control embryos reached stage 18. Embryos were fixed and processed for whole-mount in situ hybridization with the indicated probes. (A,F,K) 10–6 M AGN193109 (RAR-selective antagonist), (B,G,L) ethanol solvent control, (C,H,M) 10–6 M TTNPB (RAR-selective agonist). RAR-MO was injected unilaterally at the two-cell stage with β-galactosidase lineage tracer alone (D,I,N) or together with 1 ng XRARα2 mRNA (E,J,O). Embryos were fixed when controls reached stage 18, stained for β-galactosidase activity and processed for whole-mount in situ hybridization with the indicated probes. Some embryos were used for RNA extraction and QRT-PCR analysis as described in the text. |
