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Fig. 2. Expression of clk1 and clk3 during early development of X. tropicalis.
(A) RT-PCR analysis of temporal expression of clk1 and clk3. “-RT” indicates RNA of whole embryos (stage 20) examined in the absence of reverse transcriptase. (B–U′) Spatial expression of clk1 and clk3 analyzed by WISH in developing embryos at stages 9, 10.5, 15, 20, 23, and 30. The expression of clk1 and clk3 are shown in blue/purple. (B, D, L, and N) Animal view. (C, E, M, and O) Vegetal view. (F, H, J, P, R, and T) Dorsal view with the anterior to the left. (G, I, Q, and S) Posterior view with the dorsal to the left. (K and U) Lateral side view with the anterior to the left. (K′ and U′) Magnified view of K and U, respectively. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.) |
