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Fig. 4. Whole-mount in situ hybridization of Xerl. (A) Dorsal view (st.15). Anterior is left. Arrowhead indicates posterior part of neural groove. (B) Anterior view of (A). Arrows show anterior part of neural plate. (C) Dorsal view (st.17). Anterior is left. Arrowhead indicates anteriorly bilateral expression. (D) Anterior view of (C). Arrowheads indicate anteriorly bilateral expression. Arrow indicates hypothalamus primodia. (E) Dorsal view (st.20). Anterior is left. Arrowhead indicates neural tube. (F) Anterior view of (E). Arrowheads indicate eye anlage and future midbrain. (G) Dorsal view (st.24). Anterior is left. (H) Anterior view of (G). Arrowheads indicate same region in (D,F). (I) Dorsal view (st.28). (J) Anterior view of (I). Arrowhead indicates midbrain expression. Arrow shows diencephalic expression. (K) Xpax6 expression (st.25). (L) Anterior view of (K). White arrowhead indicates blank of expression at midbrain. (M,M′) Xpax6 expression (st.32). (N,N′) Xtll expression (st.32). (O,O′) Xerl expression (st.32). (M–O) Lateral view. (M′–O′) Highly magnified dorsal view. (P,Q) Transverse section of the whole embryo stained with Xerl probe at st.32. (P) Midbrain. (Q) Lens vesicle. Arrows indicate remarkable expression at the lens equator zone. (R) In situ hybridization on the tissue sectioned at diencephalic level (st.32). Arrowheads indicate Xerl expression near the apical surface of ventricular zone. Arrow indicates Xerl expression of outer layer of neural retina. mb, midbrain; rm, rhombomere; mhb, mid-hindbrain boundary; te, telencephalon; di, diencephalon; ey, eye; gu, gut; lv, lens vesicle; nr, neural retina; dt, dorsal thalamus; ht, hypothalamus; ls, lens. |
