| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| cxcr4 | xenopus | epidermis [+] |
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Experiment details for cxcr4
Grainyhead-like 3, a transcription factor identified in a microarray screen, promotes the specification of the superficial layer of the embryonic epidermis.
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| Fig. 1. A microarray and in situ screen to isolate genes differentially expressed between the superficial and deep cells. (A) Superficial and deep cells were isolated from early Xenopus embryos and used to make a probes which were hybridised to the microarray. The positives were then screened by in situ hybridisation to confirm that they showed differential expression. (B) Genes with superficial expression; Part 1. A wholemount and a section are shown for each gene at gastrula (left-hand panels) and neural plate stage (right-hand panels). The gastrula stage wholemounts have the animal hemisphere to the top. The sections show expression in the animal hemisphere. The chemokine receptor 4 wholemount embryo is bisected to show the expression in the involuting marginal zone. The stage 14 wholemounts are shown as lateral (L) or dorsal views (D) as indicated, with anterior to the left. The stage 14 sections are transverse and show the expression in the epidermis, except for the krupple like factor where the expression at the border of the neural plate is shown. The poliovirus receptor related 2 has expression in the deep epidermis (arrow head) as well as the superficial cell epidermis. Arrows indicate areas of expression. The genes are labelled with the top blast hit and unique EST identifier (e.g., Xl075n14). |