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Figure 4. Whole-mount in situ hybridization for Xenopus tropicalis DAZ-like gene (Xtdazl) mRNA (A-E) and transverse section of Xtdazl-stained embryo (F-I). A,F: Xtdazl mRNA-positive cells are found in the posterior and lateral regions of the endoderm at St. 23. B,G: Some cells expressing Xtdazl mRNA gradually migrated to more anterior and dorsal endodermal regions at St. 28. C,H: Xtdazl mRNA-positive cells indicated by the arrow in H are localized beneath the dorsal mesentery at St. 35. D,I: Xtdazl mRNA-positive cells indicated by the arrow in I are confined to the dorsal crest at St. 40. E: The cells indicated by the arrowheads are not spread laterally. Scale bars = 500 mu m in A-E, 200 mu m in F-I. |