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dhrs3xenopus notochord [+] 

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Experiment details for dhrs3

Kam RK et al. (2010) Assay

Developmental expression of Xenopus short-chain dehydrogenase/reductase 3.

Gene Clone Species Stages Anatomy
dhrs3.L laevis NF stage 13 notochord
dhrs3.L laevis NF stage 15 notochord

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  Fig. 3. Transverse and longitudinal sections of embryos showing dhrs3 expression. Stage 11 embryo (A) was sectioned showing the expression domain in dorsal blastopore lip and axial mesoderm (B). (C-D Transverse section from a stage 13 embryo (C), indicating the expression in notochord and neuroectoderm. The higher magnification of framed region (D) is shown in (D. (E-G Longitudinal and transverse sections from a stage 15 embryo (E) indicated dhrs3 was expressed in notochord and neuroectoderm. (H-N) Transverse sections of a stage 35 embryo (H) illustrating the expression in the midbrain, lens, otic vesicles, ventral pharynx, gut, pronephros, dorsal spinal cord and notochord. Expression pattern of rdh10 is shown in (IM. Abbreviations: bc, blastocoel; bpl, blastopore lip; ne, neuroepithelium; pm, paraxial mesoderm; nc, notochord; mb, midbrain; ov, otic vesicle; p, pharynx; pn, pronephros; sc, spinal cord.

Gene Clone Species Stages Anatomy
dhrs3.L laevis NF stage 15 notochord

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  Fig. 2. Spatial expression pattern of dhrs3. Whole mount in situ hybridization was employed to examine the spatial expression of dhrs3 at different developmental stages as indicated. (A) Lateral view of a four-cell stage embryo. (B-F Dorsal view of early stage embryos. dhrs3 transcripts were localized in the dorsal blastopore lip and the ridge of neural fold. The expression of rdh10 in stage 11, 13 and 19 are shown in C'D'F' for comparison. (G-I) Frontal view of stage 13, 15, 19 embryos, which showed that dhrs3 expression was absent in the head, but present in the posterior edge of the up-folding neural tube. (J-L) Lateral view of stage 13, 15, 19 embryos, showing dhrs3 expression in ventral bilateral regions (arrow). (M-N Lateral views of tailbud and tadpole stage embryos, showing the expression pattern of dhrs3 and rdh10. The anterior region of a stage 35 embryo showing dhrs3 expression was magnified in (N). dhrs3 expression was detected in the forebrain, midbrain, pharynx, spinal cord and pronephros (arrow). In lateral views (J-N), anterior is to the left. Abbreviations: fb, forebrain; mb, midbrain; p, pharynx; sc, spinal cord; pn, pronephros.