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Fig. 9. Expression of Xenopus dkk-1 and chicken dkk-3.
Expression of Xenopus dkk-1 (A-C) and chicken dkk-3 (D-F) was analyzed by whole mount in situ hybridisation.
(A) dkk-1 expression in early tailbud (stage 26). Dorsal view with anterior to the left. Inset shows transverse vibratome section at the level indicated by the dashed line. Expression occurs in cranial nerves (cn), eye (ey), forming somites (fs), otic vesicle (ov), proctodeum (pr). Three stripes of expression running along the dorsal trunk correspond to cells of the dorsal neural tube (dn) and cells at boundary of dorsal somite and neural tube (ds). (B, C) dkk-1 expression in tailbud (stage 32). Expression occurs in cranial nerves (cn), dorsal eye (de), dorsal otic vesicle (do), fin mesenchyme (fm), forming somites (fs) pituitary (pi), proctodeum (pr) as well as an unidentified population of cells around the pronephros that may represent pigment cells by location and shape. Transverse section at hindbrain level shows expression in archenteron roof (C). (D) Chicken embryos at stage 8 (four somites), (E) stage 15 (about 24 somites), (F) stage 21 (3.5 days, 45 somites), dorsal view. Inset: 1-2 mm thick transverse slice of spinal cord taken at hindlimb level.
Key:
ao, area opaca;
hb, hindbrain;
he, heart;
mb, midbrain;
ne, neuroectoderm;
nc, notochord;
pm, pre-somitic mesoderm;
rp, roof plate;
so, somites. |
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dkk1 (dickkopf WNT signaling pathway inhibitor 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 26, dorsal view, anterior left. Inset is a transverse section at the level indicated.
Key: ey = eye, ov= otic vesicle; dn= dorsal neural tube/roof plate; cn = cranial nerves; ds= cells at boundary of dorsal somite and neural tube; fs is rostral somite; pr= proctodeum. |
