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Figure 3. Spatial expression of Xena transcripts during Xenopus development. Whole-mount in situ hybridization was carried out using a digoxigenin-labeled riboprobe. A-F: Xena expression in stage 14 (A), stage 20 (B), stage 25 (C), stage 28 (D), and stage 35 (E,F) embryos. G-I: Transverse sections of stage 35 embryos at the level of the eyes (G), otic vesicle/hindbrain (H), and trunk (I). Nieuwenkoop-Faber stages are indicated in lower right corners. Anterior is to the left in A and B. br, brain; ey, eye; nt, neural tube; ol, olfactory placode; ot, otic vesicle; myo, myotome; no, notochord; pn, pronephros; pnd, pronephric duct; h, heart. |
