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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
enpp4
|
|
laevis
|
NF stage 5 (16-cell)
|
animal blastomere
,
animal hemisphere
|
enpp4
|
|
laevis
|
NF stage 22
|
hatching gland
,
cement gland primordium
|
enpp4
|
|
laevis
|
NF stage 32
|
otic vesicle
,
brain
,
forebrain
,
cement gland
,
somite
,
[+]
|
enpp4
|
|
laevis
|
NF stage 41
|
spinal cord
,
somite
,
pronephric duct
,
hindgut
,
axial mesoderm
,
[+]
|
enpp6
|
|
laevis
|
NF stage 5 (16-cell)
|
animal hemisphere
,
animal pole
|
enpp6
|
|
laevis
|
NF stage 37 and 38
|
late proximal tubule
,
early proximal tubule
,
pronephric tubule
|
enpp6
|
|
laevis
|
NF stage 28
|
otic vesicle
,
hindbrain
,
foregut
,
pronephric mesenchyme
,
rhombomere
|
enpp1
|
|
laevis
|
NF stage 28
|
otic vesicle
,
notochord
,
ventral blood island
|
enpp1
|
|
laevis
|
NF stage 35 and 36
|
otic vesicle
,
forebrain
,
notochord
,
ventral blood island
,
trigeminal ganglion
,
[+]
|
enpp1
|
|
laevis
|
NF stage 37 and 38
|
head region
,
dorsal aorta
,
heart
,
ventral blood island
,
posterior cardinal vein
,
[+]
|
enpp1
|
|
laevis
|
NF stage 41
|
brain
,
dorsal aorta
,
posterior cardinal vein
,
pronephric sinus
,
head vasculature
,
[+]
|
enpp7
|
|
laevis
|
NF stage 45
|
small intestine
,
external coil of the small intestine
|
|
|
Fig. 6. Spatial expression profile of enpp1, 4, 6 and 7 during development. Whole mount in situ hybridisation with DIG-labelled antisense RNA probes was performed on embryos from stages 5-41 for enpp1, 4, 6 and stages 32-45 for enpp7. All embryos were analysed from the same experimental set. The differences in colours result from clearing the embryos and photographing them against a white background to visualise staining. (A) Whole-mount in situ hybridisation analysis of enpp1 expression. Lateral view at stages 28 (i), 35/36 (ii) and 37/38 (iii). Detail of the fin at stage 41 (iv) and dorsal view of a stage 41 embryo (v). Anterior is right and dorsal is up, except in (v). da, dorsal aorta; dc, duct of cuvier; dlav, dorsal longitudinal anastomosing vessel; h, heart; pcv, posterior cardinal vein; ps, pronephric sinus; vbi, ventral blood island. (B) Whole-mount in situ hybridisation analysis of enpp4 expression. Animal view at stage 5 (i), anterior view at stage 22 (ii), lateral view at stages 32 (iii), 37/38 (iv) and 41 (v). For the lateral views, dorsal is up and anterior is right. Transverse sections at stage 37/38 through the proximal tubules (vi). Dorsal is up. The dotted line through the pronephric tubules (iii) correspond to planes of section in (vi). cg: cement gland; ct, collecting tubules; dt, distal tubules; hg, hatching gland; pt, proximal tubules; s, somites. (C) Whole-mount in situ hybridisation analysis of enpp6 expression. Lateral view at stage 5 (i), lateral view at stage 28 (ii) and 37/ 38 (iii). Dorsal is up and anterior is right. Transverse sections in the proximal tubules level at 37/38 (iv). The dotted line through the pronephric tubules correspond to planes of section in (iv). p, pronephros; pt, proximal tubules. (D) Whole-mount in situ hybridisation analysis of enpp7 expression. Ventral view of stage 45 embryos hybridized with anti-sense (i) and sense (ii) probe, lateral view of the gut region of a stage 45 embryo (iii), cryostat section (iv) at 28μm of a stage 45 embryo through the gut. no, notochord; m, muscles; sc, spinal cord; sia, first sections of the small intestine; sib, external coil of small intestine; sic, internal coil of small intestine (from Chalmers and Slack, 1998). |