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Figure 3. Spatial analyses of er71 expression. A: Whole-mount in situ hybridization of wild type embryos of different stages. er71 is expressed in progenitor cells of the vascular system. a,b: er71 expression at NF stage 17–18. er71 is expressed in the anterior portion of the embryo in the vicinity of the cement gland. c–j: er71 expression between NF stage 20 and 37. k–m: Sections of a NF stage-34 embryo show expression of er71 in endothelial structures in the retinal veins (k,l), intersomitic veins (l), branchial arches and duct of cuvier (m), and anterior aorta and vitelline veins (n). aa, anterior aorta; ap, anal porus; (ba, branchial arches; dc, duct of cuvier; h, heart anlage; isv, intersomitic veins; mnc, migrating neural crest; pcv, posterior cardinal vein; pp, posterior precursors; rv, retinal vein; vp, ventral precursors; vbi, ventral blood islands; vv, vitelline veins. |