|
FIGURE 2 | Expression of thalamic markers at early embryonic
stages 37/38. Microphotographs of whole mounts (A,N) and sagittal
(B,C,E,J,L,O,R) or transverse (D,F–I,K,M,P,Q) sections of embryos at
stages 37/38. Photographs correspond to single ISH (purple; A,I), double
ISH (purple/orange; G,N–Q) and combination of ISH (purple) with IHC
(brown) (B–E, F,H,J,K,L,M,R). The markers labeled are indicated in the
upper left of each photograph. All images are oriented following the
same standard: dorsal is upwards in transverse and sagittal sections, and
rostral is to the left in sagittal sections. The neuromeric boundaries and
main brain subdivisions are indicated to assist in the precise localization
of the labeling. At these stage c-Th and r-Th subdivision of the thalamus
were distinguished (M). The levels of the transverse sections (D,F,P) are
indicated in photographs (C,E,O), respectively. Scale bars = 100 µm
(A,B,L,N,O), 50 µm (C–K,M,P–R). See list for abbreviations. |