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Fig. 3. Whole-mount in situ expression of xFKBP. (A) Stage 10.5, vegetal view, dorsal to top. Staining is in the dorsal blastopore (arrow). (B) Transverse section through the dorsal blastopore of a stage 10.5 embryo. xFKBP expression in the mesodermal layer of the organizer (arrows). The arrowhead indicates the position of the dorsal blastopore lip. (C) Stage 12, dorsal view, anterior to top. xFKBP accumulates in the prospective notochord (arrow). (D) Transverse section through the dorsal blastopore of a stage 12 embryo. xFKBP expression is detected in the involuting mesoderm (arrows). The arrowhead indicates the position of the dorsal blastopore lip. (E) stage 13, dorsal view, anterior to top. xFKBP expression is restricted to the notochord (arrow). (G) Stage 22, lateral view, anterior to the right. Arrow indicates staining in the somites. (H) Stage 30, lateral view, anterior to right. Staining is in the epidermis, the somites (arrow) and the branchial arches (arrowhead). A control embryo hybridized with a sense probe is shown at the bottom. (I) Higher magnification of the head region of a stage 30 embryo, note the staining in the branchial arches (arrowheads). (K) Stage 35 tadpole, lateral view. (L) Higher magnification of the tail region of the embryo presented in panel (K), xFKBP expression persists in the notochord (arrow). (M) Longitudinal section through the head of the embryo presented in panel (K), anterior to right. XFKBP is detected in the mesenchyme of the branchial arches (arrowheads). (F,J) Transverse sections of a stage 13 (F) and a stage 30 (J) embryos. hy, hypochord; no, notochord; np, neural plate; nt, neural tube; ph, pharynx; so, somite. |
