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Fig. 2. Localization of Xfz7 mRNA in Xenopus embryos. Whole-mount in situ hybridization for Xfz7. Maternal Xfz7 transcripts are localized in the animal region of two-cell (A) and eight-cell embryos (B). A and B show horizontal sections through the embryo. Zygotic Xfz7 is detectable at early gastrula stages on the dorsal side, stages 10, 10.5, vegetal view (C,D). The arrows show staining at the dorsal lip. (E) Stage 11, mid-gastrula, dorsal is on top. The Xfz7 expression domain extends to form a ring around the closing blastopore. (F) Stage 19, neurula, dorsal view. Xfz7 expression is seen at dorsal and anterior positions. (G,H,I) In tailbud stages (26, 28 and 31), Xfz7 is expressed preferentially in the head region, the pronephros and the tailbud. (J) In stage 35/36 tadpoles, no Xfz7 expression can be detected in the pronephros and tail, whereas the expression in the head persists. |
