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Figure 2. Localization of GAP43 in Zebrafish and Xenopus myotubes has a periodic sarcomeric spacing. Representative immunofluorescence images of muscle cell cultures deriving from Zebrafish (A) and Xenopus (B) samples. Confocal microscopy analysis shows a single striation cross-orientated as indicated by arrowheads (A and B). C) Measurements of distances between striations. Scale bars: A) 20 µm; B) 2 µm. |
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Figure 3. GAP43 is localized close to CRUs, in adult fibers. Representative immunofluorescence images of double-immunolabeled muscle fibers from Zebrafish (A-C) and Xenopus (D-F) samples. In adult Zebrafish and Xenopus muscle fibers, the staining of CRUs (marked with an anti-RyR antibody, B and E) forms dotted single rows along the Z-lines. Also GAP43 staining produces a single dotted cross-striation (A and D), which appears to co-localize with that of RyR (C and F, merged images). Scale bars: 5 µm. |
