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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
gnb1
|
|
laevis
|
unfertilized egg stage
|
animal
,
animal pole
|
gnb1
|
|
laevis
|
NF stage 2 (2-cell)
|
animal
,
animal pole
|
gnb1
|
|
laevis
|
NF stage 8
|
animal hemisphere
,
animal
|
gnb1
|
|
laevis
|
NF stage 14
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
|
gnb1
|
|
laevis
|
NF stage 28
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
spinal cord
,
[+]
|
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Fig. 5. Expression pattern of XGB1 in Xenopus observed after whole mount in situ hybridization. In situ hybridization was carried out as described in
Section 4. (A-C) Cleared oocyte or embryos are positioned with the animal pole on top. They correspond, respectively, to mature oocyte, stage 2 embryo
and morula. Note the restriction of the labeling to the animal pole. (D) Vegetal view of a gastrula. The staining is mainly localized in the dorsal
region under the blastopore lip (bl), while the vegetal pole is unstained. (E) Dorsal view of a neurula showing specific labeling on the neural plate. (F)
Early larval stage; head is on the left, dorsal on the top. An intense staining is observed in neural structures (br, brain; sp, spinal cord) and eye (e). All
embryos were depigmented after in situ hybridization. |
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Fig. 6. Transverse sections from whole mount in situ hybridized embryos shown in Fig. 5. (A-C) These transverse sections arc presented with the
animal pole on the top, and correspond, respectively, to mature oocyte, stage 2 embryo. and morula. Note that the staining is confined to the animal
pole. (D) Section of a gastrula showing a specific labeling above the dorsal blastopore lip (dbl) in the ectoderm. (E) Transverse section of neurula
showing the neural plate (np) staining. (F) Transverse section through the trunk of an early larva stage showing the labeling of the neural tube (nt).
Abbreviations: gv, germinal vesicle; blc, blastocoele: not, notochord. |