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Display additional annotations [+]
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Fig. 6. mRNA expression of GPAT1 and GPAT2 in different organs detected by whole- mount in situ hybridization. (A) GPAT1 is expressed during kidney and liver organogenesis. Whole-mount in situ hybridization with the GPAT1 antisense riboprobe of embryos at the indicated stages is shown in the left column. Higher magnifications of the kidney and liver regions are shown in a lateral view in the right two panels (anterior to left). Eye (E), kidney (K) (early expression detected at stage 26) and liver (L) (early expression detected at stage 33/34) are shown. (B) GPAT1 and GPAT2 show a different expression pattern at stage 40. GPAT1 and GPAT2 are both expressed in the nervous system (E, eye; B, brain; and SC, spinal cord). Further, GPAT1 is strongly expressed in liver (L) and kidney (K), whereas GPAT2 is expressed in presumptive gonad (testis-T) and weakly in kidney. |
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gpam ( glycerol-3-phosphate acyltransferase, mitochondrial ) gene expression in Xenopus laevis embryos, NF stage 26, as assayed by in situ hybridization, lateral view, anterior left, dorsal up. |
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gpam ( glycerol-3-phosphate acyltransferase, mitochondrial ) gene expression in Xenopus laevis embryos, NF stage 28, as assayed by in situ hybridization, lateral view, anterior left, dorsal up. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
gnpat
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laevis
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NF stage 33 and 34
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otic vesicle
,
forebrain
,
head region
,
eye
,
pronephric kidney
,
[+]
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gnpat
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laevis
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NF stage 37 and 38
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otic vesicle
,
head region
,
pronephric duct
,
eye
,
pronephric kidney
|
gpat2
|
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tropicalis
|
NF stage 33 and 34
|
otic vesicle
,
brain
,
spinal cord
,
head region
,
somite
,
[+]
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gpat2
|
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tropicalis
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NF stage 37 and 38
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brain
,
spinal cord
,
head region
,
somite
,
pronephric duct
,
[+]
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gpat3
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laevis
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NF stage 33 and 34
|
otic vesicle
,
forebrain
,
hindbrain
,
spinal cord
,
head region
,
[+]
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gpat3
|
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laevis
|
NF stage 37 and 38
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forebrain
,
hindbrain
,
head region
,
pronephric duct
,
liver
,
[+]
|
gpat4
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laevis
|
NF stage 33 and 34
|
otic vesicle
,
forebrain
,
hindbrain
,
spinal cord
,
pronephric duct
,
[+]
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gpat4
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laevis
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NF stage 37 and 38
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otic vesicle
,
spinal cord
,
head region
,
pronephric duct
,
liver
,
[+]
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Fig. 5. mRNA expression of GPATs and DHAPAT in embryos and in kidney between stages 32 and 37/38 as detected by whole-mount in situ hybridization. mRNA for all GPATs and DHAPAT are present in the kidney (K), as well as in the nervous system, including the eye (E), otic vesicle (OV), spinal cord (SC) and branchial arches (BA). GPAT1 and GPAT4 are also expressed in the liver (L). Lateral views at the indicated stages (anterior to left). GPAT1, GPAT2, GPAT4, and DHAPAT are expressed in the pronephric tubules (arrows) and ducts (arrowheads), but GPAT3 is not expressed in the pronephric ducts (white arrowhead). |
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gpam ( glycerol-3-phosphate acyltransferase, mitochondrial ) gene expression in Xenopus laevis embryos, NF stage 32, as assayed by in situ hybridization, lateral view, anterior left, dorsal up. |
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Display additional annotations [+]
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Supplementary Figure 3. mRNA expression of GPAT1 in the liver. GPAT1 is expressed in kidney (K), eye (E), otic vesicle (OV), spinal cord (SC) and liver (L), as confirmed with an antisense riboprobe against the liver specific marker AMBP. |
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gpam ( glycerol-3-phosphate acyltransferase, mitochondrial ) gene expression in Xenopus laevis embryos, NF stage 35 and 36, as assayed by in situ hybridization, lateral view: anterior left, dorsal up. |
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gpam ( glycerol-3-phosphate acyltransferase, mitochondrial ) gene expression in Xenopus laevis embryos, NF stage 37 and 38, as assayed by in situ hybridization. Lateral view: anterior left, dorsal up.
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gpam ( glycerol-3-phosphate acyltransferase, mitochondrial ) gene expression in Xenopus laevis embryos, NF stage 40, as assayed by in situ hybridization, lateral view, anterior left, dorsal up. |