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Fig. 1. Expression patterns of Gli3, dHAND, Shh, Ptc1, Ptc2 and Gli1 in a stage 53 hindlimb bud (A–C), stage 53 limb blastema (D–F, J–L) and froglet blastema (G–I, M–O). (A–C) In the stage 53 hindlimb bud, Gli3 was expressed broadly without a posterior margin (dotted line in panel A). dHAND was expressed in the posterior part (B), and the Shh-positive domain was limited to the posterior margin (C). (D–F) In stage 53 blastema at 4 days after amputation, Gli3 (D) and dHAND (E) expressions were observed in the anterior mesenchyme and posterior mesenchyme, respectively. Strong expression of Shh (arrowheads in panel F) was found in the posterior mesenchyme. (G–I) In the froglet blastema, Shh expression was not detected (I), although expressions of Gli3 (arrowheads in panel G) and dHAND (H) were detected. (J–L) In stage 53 blastema, signals for Ptc-1 (J), Ptc-2 (K) and Gli1 (L) were detected in the posterior mesenchyme. (M–O) In contrast, signals for these three genes were undetectable in the froglet blastema at 10 days. All sections are transverse sections, and the anterior side is on the left of all panels. Lines indicate the amputation plane. Scale bars = 400 μm for stage 53 (A–F, J–L) and 200 μm for froglet (G–I, M–O). |
