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Fig. 5. (A) Developmental expression of XKv1.2′, XKv1.4 and XKv1.10 in whole embryos. cDNA was prepared from whole embryos at stages 2, 8, 10.5,11.5, 12.5, 14, 17, 21, 32 and 50 and XKv1.2′, XKv1.4 and XKv1.10 expression was assessed using specific primers as described in the Materials and methods. (B) Expression of XKv1.2′, XKv1.4 and XKv1.10 in muscle and nerve. cDNA was prepared from the muscle of stage 14 and 21 embryos, adult sartorious muscle and stage 21 spinal cord. Expression of XKv1.2′, XKv1.4 and XKv1.10 RNA was assessed using specific primers as above. Additional RT–PCR with primers specific for medium weight neurofilament (NFM) was carried out to demonstrate that the muscle RNA sample was uncontaminated by nerve. (Controls) RT–PCR with primers for H4 histone was carried out with each of the cDNA preparations as a positive control. The control lane at the right of the figure for the Kv1.2′, Kv1.4, Kv1.10 and H4 reaction contained no template. The H4 primers were used with RT-minus template to ensure there was no genomic DNA and the control lane for this series contained stage 50 cDNA. |
