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Figure 1. ME LK localization in epithelial cells of Xenopus gastrula embryos. (A) Indirect immunofluorescence with anti-MELK antibodies. Endogenous
MELK is detected at the cell cortex in interphase cell (arrowhead) and further accumulates at the cell cortex at the metaphase to anaphase transition
(m: metaphase, a: anaphase). The yellow line on the scheme at the bottom indicates the position of the confocal plane relative to the cell surface. DN A
(blue) which is situated more profoundly in the cells was merged with overlaying ME LK signal. (B and C) The mRNA coding for GFP tagged ME LK inactive
mutant (GFP-MELK K/R) which does not induce cytokinesis failure was microinjected in an embryo at the two cell stage and GFP-MELK K/R was
followed in a live gastrula embryo. Two confocal planes taken at the same time are shown. GFP-MELK K/R is localized at the cell cortex of interphase
cells (i: interphase cell, arrowhead in b and c) and further accumulates at the cell cortex in cytokinetic cell (cyt). Note that the apical domain of the
cytokinetic cell is beginning its division whereas its baso-lateral membrane is already divided. * and ** indicate the two forming daughter cells to
facilitate analysis of the original figure and the related scheme at the bottom. |
