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Figure 2. Stromelysin-3 (ST3) and collagenase-4 (Col4) have distinct spatiotemporal expression profiles during embryogenesis. Whole-mount in situ localization of ST3 (A,C) and Col4 (B,D) mRNA showed very restricted expression patterns during development at stages 32 (A,B) and 45 (C,D). ST3 was largely restricted to the branchial arches (A, single arrow) and dorsal posterior endoderm (A, double arrow) at stage 32 and to structures in the thorax (C, t) and the dorsal anterior axis (C, d) at stage 45. Col4 could not be detected at stage 32 (B) and was only detectable at approximately stage 45 where it was also present in the thorax (D, t) and the dorsal anterior axis (D, d), although in a somewhat different pattern compared with ST3. |
