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Fig. 1. (A) Comparison of the predicted amino acid sequence of Xmxi1 with the mouse Mxi1 isoforms. GenBank accession number Xmxi1: DQ137875. (B) RT-PCR analysis of Xmxi1 at different stages of development. Histone H4 was used as a loading control. (C) Whole-mount in situ hybridization expression analysis of Xmxi1 in comparison with X-ngnr-1. (D–F) Double labeling of Xmxi1 and Sox3 expression. Sox3, red; Xmxi1, dark blue. (D) Stage 14, anterior view. (E) Stage 14, dorsal view. White arrowhead marks the lateral stripe of Xmxi1. (F) Transversal section of E. (G–N) Spatial expression of Xmxi1 during later stages of Xenopus development. (G) Stage 13, dorsal, anterior up. (H) Stage 16, dorsal view, anterior left. (I) S1 transversal section. (J) Stage 22, lateral. (K) Stage 27, anterior (L) Stage 37, lateral. (M) S2 transversal section. (N) S3 transversal section, dark brown staining at the roof of the spinal chord is pigmentation not Xmxi1 staining. el, epithelial layer; hb, hindbrain; i, intermediate; iz, intermediate zone; l, lateral; le, lens; m, medial; mb, midbrain; n, notochord; op, olfactory placode, r, retina; sl, sensory layer; st, stage; tg, trigeminal placodes; vz, ventricular zone. |
