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Figure 1 c-myc and n-myc are expressed sequentially during early development and in the CMZ. A: c-myc expression from stage 11/12-19. E: n-myc expression from Stage 139. I,J: c-myc expression in the CMZ as visualized on wholemount and tissue sections of stage 41/42 embryos. M, N: n-myc expression in the CMZ on wholemount and tissue sections of Stage 41/42 embryos. K: Double in situ hybridization in stage 32 embryos with c-myc visualized using BM Purple and n-myc visualized using ELF 97. O: Double in situ hybridization in the post-embryonic retina with c-myc visualized using ELF 97 and n-myc visualized using BM Purple. L, P: c-myc and n-myc double in situ hybridization on tissue sections with c-myc visualized using BM Purple and n-myc visualized using Fast Red. Fluorescent images were modified in single channels. Dashed rectangles in the upper panels encompass the region of the eye field. Dashed areas in the lower panels encompass the CMZ regionsn wholemount views (L,K,M,O), this is the annulus created by the two dotted circles. We have not identified the structures outside the eye regions that label with c-myc or n-myc. Bars: A-H 1 mm; I, M 100 lm; J, N 100 lm; K 250 lm; O, L, P 100 lm. See also Figure S1.
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