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myo10xenopus lens 

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Experiment details for myo10

Hwang YS et al. (2009) Assay

Myosin-X is required for cranial neural crest cell migration in Xenopus laevis.

Gene Clone Species Stages Anatomy
myo10.L laevis NF stage 33 and 34 lens
myo10.L laevis NF stage 37 and 38 lens

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  Figure 1. Spatial and temporal expression of MyoX in Xenopus. A: Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. MyoX mRNA was detected at all embryonic stages. B-H: Whole-mount in situ hybridization (ISH); stages are as follows: St. 16 neurula (B,C), St. 19 neurula (D), St. 21 tail bud (E), St. 24 tail bud (F), St. 33 tadpole (G), and St. 38 tadpole (H). Panel (C) is a transverse section of the embryo in panel (B). Early MyoX expression is most abundant in cranial neural crest (CNC), trunk neural crest (TNC), paraxial mesoderm (PM) and the anterior neural plate border (NPB). D-G: Later, strong expression is visible in placodes. In the tadpole, MyoX signal is elevated in cranial nerve V sim X, termini of nerve IX, X (arrowheads) and three termini of nerve VII (arrows, also see Supp. Fig. S3), and in epibranchial placodes (asterisks, G). aB, anterior branchial arch; Br, branchial arch; F, forebrain; H, hindbrain; Hy, hyoidal arch; IX, glossopharyngeal nerve; L, lens placode; M, midbrain; Md, mandibular arch; No, notochord; So, somites; pAD, anterodorsal placode; pAV, anteroventral placode; pB, posterior branchial arch; pM, middle lateral line placode; pOI, olfactory placode; pP, posterior lateral line placode; pPr, profundal placode; pV, trigeminal placode; V, trigeminal nerve; Vmd, mandibular branch of trigeminal nerve; VII, facial nerve; vOt, otic vesicle; Xb, branchial branch - cranial nerve X; Xl, lateral branch - cranial nerve X; Xv, visceral branch - cranial nerve X.