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myt1xenopus dorso-lateral 

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Experiment details for myt1

Vernon AE et al. (2003) Assay

The cdk inhibitor p27Xic1 is required for differentiation of primary neurones in Xenopus.

Gene Clone Species Stages Anatomy
myt1.S laevis NF stage 11.5 dorso-lateral
myt1.S laevis NF stage 13 dorso-lateral

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  Fig. 1. p27Xic1 is expressed in cells destined to become primary neurones. Whole-mount in situ hybridisation at indicated stages show expression of p27Xic1 (A-C,G,I) or XMyT-1 (D-F,H). Except for G, figures show a dorsal view with anterior towards the bottom. Both p27Xic1 and XMyT-1 are found in lateral, intermediate and medial stripes of primary neurones, placodes (arrows) and the trigeminal ganglia (arrowheads). (G) Stage 13 embryos show p27Xic1 staining in the epidermis. (H) Double in situ hybridisation demonstrates overlap between p27Xic1 (purple) and XMyT-1 (light blue). (I) Vibratome section of a stage 15 embryo shows p27Xic1 expression in the myotome (My), notochord (Not) and primary neurone precursors (PNP) in the sensorial layer of the neural plate (arrow).

Gene Clone Species Stages Anatomy
myt1.S laevis NF stage 18 dorso-lateral

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  Fig. 2. Embryos depleted of p27Xic1 protein fail to produce differentiated primary neurones. (A) Western blot for endogenous p27Xic1 protein levels on stage 18 embryos that were injected with 20 ng Con Mo or p27Xic1 Mo. Cytoskeletal-β-tubulin demonstrates equal loading. Con Mo injection has no effect on any of the neural markers examined (B, data not shown). (C-F) p27Xic1 Mo-injected embryos were analysed for expression of X-NGNR-1, XMyT-1, NeuroD and Nβtub (purple) by whole-mount in situ hybridisation, injected side towards the left (β-gal, light blue). Injection of 20 ng p27Xic1 Mo has no effect on X-NGNR-1 (C) but ablates XMyT-1 (D), NeuroD (E) and Nβtub (F) expression.