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FIG. 4. Comparison of Xath5 and other proneural gene expression patterns in stage 40 Xenopus retina. (A) Comparison of Xath5 and neuroD expression patterns. Double in situ hybridizations were performed as in Fig. 1 with neuroD in deep purple and Xath5 in red. (A) neuroD expression is seen in cells in the CMZ and in the central retina, in the outer part of the inner nuclear layer, and in the outer nuclear layer. (B) Xath5 expression is detected in the CMZ except in cells in the most peripheral region. C and D show a higher magnification of the CMZ from the sections depicted in A and B, respectively. (E) Double labeling demonstrates that Xath5 and neuroD expressions start at the same level in the CMZ. Scale bar in A and B, 100 ﰂm; scale bar in C, D, and E, 30 ﰂm. (F) Comparison of ATH-3 and Xath5 expression patterns. Double in situ hybridizations were performed as in Fig. 1 with ATH-3 in deep purple and Xath5 in red. (F) ATH-3 expression is seen in cells in the CMZ and in the central retina, in the outer part of the inner nuclear layer. (G) Xath5 expression is detected in the CMZ except in cells in the most peripheral region. H and I show a higher magnification of the CMZ from the sections depicted in F and G, respectively. (J) Double labeling demonstrates that Xath5 and ATH-3 expressions start at the same level in the CMZ. Scale bar in F and G, 100 ﰂm; scale bar in H, I, and J, 30 ﰂm. (K) Comparison of X-MyT1 and Xath5 expression patterns. Double in situ hybridizations were performed as in Fig. 1 with this time X-MyT1 in deep purple and Xath5 in red. (K) X-MyT1 expression is seen in cells in the CMZ and in the central retina, in the inner nuclear layer. (L) Xath5 expression is detected in the CMZ except in cells in the most peripheral region. In the central retina, similarly to what was observed for X-Notch-1 and X-Delta-1, a few cells, probably late born cells, express Xath5 in this retina which is at a slightly younger stage than the retina shown in Fig. 3. M and N show a higher magnification of the CMZ from the sections depicted in K and L, respectively. (O) Double labeling demonstrates that Xath5 and X-MyT1 expressions start at the same level in the CMZ. Scale bar in K and L, 100 um; scale bar in M, N, and O, 30 um |
