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Fig. 1. Xenopus Nkx2-5 is expressed in the spleen in late embryos and adult frogs.
(A) By RNase protection, XNkx2-5 transcripts are detected abundantly in adult spleen and also in adult heart. (B,C) In situ hybridization indicates that Nkx2-5 transcripts are localized to a small crescent on the dorsal side of the stomach at stage 43, the established position of the developing spleen (Nieuwkoop and Faber, 1994). (A) RNase protection analysis of Nkx2-5 and control EF-1α transcripts in adult tissues. (B) Ventral view of a stage 43 tadpole assayed for Nkx2-5 transcripts. (C) Side view of the embryo in B. g, gut tube; h, heart; k, kidney; li, liver; lu, lung; p, pancreas; sk, skeletal muscle; sp, spleen; st, stomach. |
