| |
Fig. 2. Expression of xPAK1
mRNA and protein. (A) Northern
blot of xPAK1 mRNA during
early development. The positions
of 18S and 28S rRNAs are indicated
and the ethidium bromide
stained 18S band shown below
the Northern blot. (B) Quantitative
RT-PCR of xPAK1 mRNA from
embryos confirms the quantitation
by Northern blot and in (D) shows
the relative expression of mRNA
in isolated adult tissues. For a
given RNA source three gel tracks
are shown corresponding to PCR
reactions with 2 fold increasing
aliquots of reverse transcribed
RNA. Western analyses of endogenous
xPAK1 protein showing
its relative abundance (C) during
early development and (D) in
isolated adult tissues. In (D) the
relative abundance of xPAK1p is
indicated as �-�, �+�, etc, and �?�
indicates an unidentified peptide
present in liver. |
