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Fig. 3. Expression of Pitx genes in Xenopus tadpoles. Co-localization of Pitx1 and Pitx2c mRNA was found in the cement gland (black arrow; (A,C,E,G)). All three genes were co-expressed in the pituitary and stomodeum (yellow arrows; (A–C), (E–G)) and in the midbrain (E–G). Exclusive expression of Pitx1 was found in the foregut (blue arrow; (A,D,E,H)), lens (D+E), and posterior lateral plate mesoderm (pLPM). (C) and (G) show the asymmetric expression of Pitx2c in the left LPM and heart anlage, where Pitx1 and Pitx2b were not detected (B,D–F). Lines indicate approximate levels of sections. aLPM, anterior LPM; em, eye muscle; ha, heart anlage; hm, head mesenchyme; le, lens; LPM, lateral plate mesoderm; pLPM, posterior LPM. |
