|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
pax6
|
|
laevis
|
NF stage 42
|
retina
,
eye
,
cornea
,
retinal ganglion cell layer
,
amacrine cell
,
[+]
|
prox1
|
|
laevis
|
NF stage 42
|
lens
,
retina
,
eye
,
lens fiber cell mass
,
horizontal cell
|
vsx1
|
|
laevis
|
NF stage 42
|
lens
,
retina
,
eye
,
lens fiber cell mass
,
bipolar neuron
|
rho.2
|
|
laevis
|
NF stage 42
|
retina
,
eye
,
photoreceptor layer
,
eye photoreceptor cell
|
|
|
Figure 3. Loss of retinol binding protein 1 leads to disordered retinal layers. Transversal sections of retinol binding protein 1 (Rbp1)- or control morpholino oligonucleotide (MO)-injected embryos at stage 42. Rbp1 knockdown led to deformed and disorganized retinal layers, including rosette-like structures (close-up views) and altered retinal pigmented epithelium (RPE; red arrowheads), as shown by marker gene expression. Four of the 9 embryos showing rho expression (photoreceptor cells), 4 of the 7 embryos showing prox1 expression (horizontal cells), 5 of the 7 embryos showing vsx1 expression (bipolar cells), 6 of the 10 embryos showing pax6 expression (amacrine and ganglion cells) and 2 of the 4 embryos showing pou4f1 expression (ganglion cells) had an altered phenotype, as depicted. In contrast, the uninjected side of the embryos showed no changes of retinal lamination. Scale bar: 100 μm. Abbreviation: MO, morpholino oligonucleotide |