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Fig. 4. Cryostat sections of Xenopus retina immunolabeled with polyclonal antibody against Lam1 (A�D and F: green labeling); monoclonal antibody against h1-
integrin (A�G: red labeling) and polyclonal antibody against aPKC (H�K, red labeling). Control side (A, D, E, H, I) and injected side (B, C, F, G, J, K) of retinas at
stage 32. The injected side in panels J and K was traced by GFP. Laminin and integrin were co-detected (yellow signal) in the Bruch�s membrane (BrM) at the bases
of pigmented epithelium (PE), at the border of vitreal cavity in the inner limiting membrane (ILM) and in the lamina surrounding the lens. Severe disorganization of
these laminae was detected in the injected sides of morphants (arrows in panels B, C, F). Integrin staining, which showed a radial-like pattern in the control sides (A,
E) was affected in the injected sides of morphants (B, C, G). High magnifications (D�G) highlight the histology of the retina at its most marginal side near the lens.
Localization of aPKC at the apical side of the retinal neural precursors (nrp), underneath the pigmented epithelium (PE) was visible either in control (H, I) or in Moinjected
eye (J, K). At higher magnification, the aPKC staining of retinas in the control (I) and injected (K) sides was comparable. |
