| |
Fig. 4. Neurons and glial cells
in the regenerated telencephalon.
The distribution of neurons and
glial (ependymal) cells was
observed by immunohistochemistry
in intact froglets (A,B,E,F,J)
and froglets with regenerated
telencephalon 3 months after the
operation during the larval stage
(C,D,G,H,K). Positive signals to
anti-NeuN antibody indicate
neurons (especially nuclei) (A–E,G);
positive signals to anti-β-tubulin
I + II antibody indicate neurons
(especially axons) (F, H); positive
signals to anti-GFAP antibody
indicate radial projections of
ependymal cells (J,K). Mitral/
tufted cells had large cell bodies
and the NeuN-positive process in
regenerated telencephalon (C),
as was observed in intact froglets
(A). In the granule cell layer, small
dense cell bodies were crowded
in both the regenerated (D) and intact froglets (B). Pyramidal cells had large nuclei and extended the β-tubulin-positive process
peripherally in both regenerated (G,H) and intact froglets (E,F). Ependymal cells had radial projections in both the regenerated (K)
and intact telencephalon (J). Ependymal layers in opposition to each other were observed touching each other because the lateral
ventricle was lost by artifact (J,K). Schematic diagrams of the distribution were drawn for neurons (I) and ependymal cells (L). Bars, 20 μm. |
