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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
pou3f2
|
|
laevis
|
NF stage 18
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
|
tbx15
|
|
laevis
|
NF stage 18
|
pre-chordal neural plate
,
anterior neural fold
,
neural fold
,
neural plate border
|
tlx1
|
|
laevis
|
NF stage 32
|
otic vesicle
,
cement gland
,
profundus ganglion
,
trigeminal ganglion
,
primary heart field
,
[+]
|
hes2
|
|
laevis
|
NF stage 14
|
neural fold
,
chordal neural plate border
,
neural plate border
,
posterior placodal area
|
sox21
|
|
laevis
|
NF stage 18
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
|
isl2
|
|
laevis
|
NF stage 14
|
anterior placodal area
,
anterior neural ridge
,
neurogenic placode
|
lhx5
|
|
laevis
|
NF stage 14
|
pre-chordal neural plate
,
anterior placodal area
|
emx1
|
|
laevis
|
NF stage 28
|
forebrain
,
telencephalon
|
gfi1
|
|
laevis
|
NF stage 32
|
otic vesicle
,
ventral blood island
,
lateral line ganglion
,
posterior ventral blood island
|
mab21l2
|
|
laevis
|
NF stage 18
to
NF stage 19
|
lens placode
,
preplacodal ectoderm
|
crem
|
|
laevis
|
NF stage 32
|
neural crest
,
cranial neural crest
|
hes5.7
|
|
laevis
|
NF stage 18
|
chordal neural plate
,
anterior neural fold
,
neural fold
,
chordal neural plate border
,
anterior placodal area
,
[+]
|
hes5.4
|
|
laevis
|
NF stage 18
|
chordal neural plate
,
anterior neural fold
,
neural fold
,
chordal neural plate border
,
anterior placodal area
,
[+]
|
pou4f4
|
|
laevis
|
NF stage 14
|
presomitic mesoderm
,
chordal neural plate border
,
posterior placodal area
,
caudal presomitic mesoderm
|
tbx6
|
|
laevis
|
NF stage 18
|
chordal neural plate
,
presomitic mesoderm
,
chordal neural plate border
,
caudal presomitic mesoderm
|
ripply3
|
|
laevis
|
NF stage 14
|
chordal neural plate border
,
posterior placodal area
|
fosb
|
|
laevis
|
NF stage 32
|
head region
,
neural crest
,
cranial neural crest
,
head
|
atoh1
|
|
laevis
|
NF stage 28
to
NF stage 32
|
otic vesicle
,
hindbrain
,
anterior lateral line placode
|
|
|
Figure 4.
Expression of selected presumptive direct targets of Six1/Eya1 in whole-mount Xenopus embryos.
Genes expressed at neural plate stages (stages 14–18) are shown in panels A–L, and those only expressed at later stages are shown at mid/late tail bud stage (stages 28–32) in panels M–T. Several of the genes surveyed (Lhx5, Pou3f2b, Tbx15, Tbx6, Emx1.2 and Sim1 (A–D, M and N), were not expressed in the PPE, nor any placodal derivatives in later stages. Instead, such genes were expressed in the adjacent neural folds (Lhx5, Pou3f2b and Tbx15), ectoderm (Tbx6), or in the forebrain at later stages (Emx1.2). Several other genes were expressed broadly across the cranial ectoderm, at least partially overlapping with the PPE at neural plate stages (Ripply3, Crem, FosB and Znf214; E,O–Q), some of which are also maintained in placodal derivatives such as Znf214 in the otic vesicle (Q). The remaining genes (F–L, R–T) are expressed in parts of the PPE and maintained in some placodes (Hes2, Hes8, Hes9, Mab21l2b, Sox21, Isl2, Pou4f1.2, and Tlx1) or are expressed in a subset of placodes only (Atoh1, Gfi1a) (see Figure 4—figure supplements 1–4 for additional stages). Yellow arrows mark placodal expression. Arrowheads mark non-placodal expression. Abbreviations: pA: anterior placodal region; pAD: anterior lateral line placode; pE: epibranchial placode; pL: lens placode; L: lens; pM: middle lateral line placode; pO: olfactory placode; pP: posterior placodal region; pPrV: profundal/trigenimal placodes; vOt: otic vesicle. Plots U and V show qPCR after Six1 or Eya1 overexpression. Log2 fold change values were calculated from qPCR data obtained after overexpression of Six1-GR (U) or Eya1-GR (V) in placodal explants and are shown next to corresponding fold change values obtained from the RNA-Seq data. In all cases shown, qPCR values broadly corroborate those from the RNA-Seq data - showing up-regulation of target genes after either Six1 or Eya1 overexpression. Vertical error bars show the standard deviation of the mean of biological triplicates.
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