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smad9xenopus notochord [+] 

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Experiment details for smad9

Beck CW et al. (2001) Assay



Gene Clone Species Stages Anatomy
smad9.S laevis NF stage 25 notochord

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  FIG. 1. Endogenous BMP activity in the tail bud. (A) Embryos bisected along the anteriorposterior axis and stained using an antibody that recognizes the activated form of Smad. Strong anti-phospho-Smad1/5/8 staining is seen in the region immediately ventral to the future tail bud (black arrows), correlating to the expression of BMP4 mRNA. Nuclear phosphorylated BMP Smads are also present at lower levels in the tail-forming region itself from stage 17 on. (A) stage 12, (B) stage 15, (C) stage 17, (D) stage 20, (E) stage 25. All embryos are oriented with anterior to the left and dorsal uppermost. (F) In situ hybridization to BMP4 to show sites of endogenous transcription. (F) BMP4 expression just anterior to the ventral and lateral lips of the blastopore (black arrow). Embryo viewed from the vegetal side with dorsal uppermost. (G) Expression in embryos bisected after in situ hybridization; arrow points to strong expression in the region immediately ventral to the tail bud. Orientation is anterior to the left and dorsal uppermost. (G) stage 14, (H) stage 20, (I) stage 25. ftb, position of future tail bud; tb, tail bud; bp, blastopore.