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Fig. 4. (Panel A) Lateral view of the stage 33/34 larvae stained by whole-mount in situ for XSOX7 reveals that XSOX7 RNA is still present in the posterior cardinal veins (pcv), hindbrain (hb), and aortic arches (aa). Staining is also visible the branching intersomatic arteries (ia), epithelial straps (es), olfactory pit (op), and stomodeal depression (sd) and the notochord (not). XSOX7 is still present in ciliated cells (cc). (Panel B) In a front view of a stage 33/34 embryo, it is possible to see more clearly that XSOX7 is expressed in the stomodeal depression, the olfactory pit and in the ‘V-shaped’ epithelial straps. The position of the cement gland (cg) in marked. (Panel C) A ventral view of an embryo reveals the strong expression of XSOX7 in the epithelial straps, while a dorsal view (Panel D) reveals the strong staining of regions of the hindbrain. (Panel E) A cross section through the hindbrain region of a stained larvae reveals presence of XSOX7 RNA in the aortic arches and the outer edges of the rhombencephalon. |
