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Fig. 3. XSPR-1 and XSPR-2 expression during early embryogenesis as detected by whole-mount in situ hybridization. Antisense RNA probes used for in situ hybridization and stages of embryogenesis (Nieuwkoop and Faber, 1967) are shown on the right and on the left of each panel, respectively. (A,C,E,G) Vegetal view; (B,D,F,H) parasagittal section; (C,D) double staining in situ hybridization of Xbra (red) with XSPR-1 (purple); (D) parasagittal section of the embryo shown in (C); (G,H) double staining in situ hybridization of Xbra (red) with XSPR-2 (purple); (H) parasagittal section of the embryo shown in (G); (I) double staining in situ hybridization of Krox20 (red) with XSPR-1 (purple), anterior view; (J) anterior transversal section; (K) double staining in situ hybridization of En2 (red) with XSPR-1 (purple), anterior view; (L) lateral view of the head of a tadpole embryo; (M) double staining in situ hybridization of Krox20 (red) with XSPR-2 (purple), dorso-anterior view; (N) XSPR-2, posterior transversal section; (O) double staining in situ hybridization of En2 (red) with XSPR-2 (purple), dorso-anterior view; (P) lateral view of a tadpole embryo; (Q) transversal section at the level of the otic vesicle; (R) transversal section at the level of the midbrain/hindbrain boundary; (S) parasagittal section through the tail tip region. Abbreviations: aet, archenteron; bc, blastocoel; br, blastocoel roof; cnh, chordaneural hinge; end, endoderm; ep, epidermis; ev, eye vesicle; eym, endodermal yolk mass; fb, forebrain; lm, lateral mesoderm; mbh, midbrain/hindbrain boundary; mc, mesencephalon; nc, notochord; nec, neuroenteric canal; ov, otic vesicle; pw, posterior wall; rb, remnants of blastocoel; rc, rhombencephalon; rv, rhombencephalic ventricle; s, somites; sln, sensorial layer of neuroectoderm; sm, somitogenic mesoderm; vym, vegetal yolk mass. |
