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Supplementary Fig. 2 Filopodia interact directly with epithelial vertices. a, 3D rendering of integrating MCC during the probing phase. The MCC (expressing α-tubulin::LifeAct-RFP, pseudo-colored in green) uses filopodia to attach to the vertices of epithelial goblet cells (expressing nectin::LSR-RFP, pseudo-colored in magenta). White arrowheads depict filopodia-vertex contact and yellow arrows depict contact retraction. b, 3D rendering from Fig. 2e of filopodium pulling on vertex (marked by white arrow). White dotted line outlines the cortex of integrating MCC and the yellow dotted line outlines the top of the superficial epithelium. c, Epithelial vertex-pulling quantification. Average MCC F-actin intensity (green) and vertex length (purple) during pulling and retraction. T=0 marks the vertex length maxima. Data show mean ± SEM (n=9 pulling events from 3 embryos from N=3 experiments). d, Orthogonal (XZ) projections of integrating MCC (expressing α-tubulin::LifeAct-RFP, pseudo-colored in green) expressing LSR-3xGFP (pseudo-colored in magenta). LSR-3xGFP is recruited to the contact points between the MCC and the vertex (white arrows, t=0 min and t= 10 min) and to filopodia (orange arrows, t=8 min and t=11 min). Yellow boxes mark insets with separate channels. Scale bar: 5 μm. e, Orthogonal (XZ) projections of integrating MCC expressing α-tubulin::LSR-GFP (pseudo-colored in green) interacting with vertices labeled with LSR-RFP expressed under nectin promoter (pseudo-colored in magenta). Yellow arrowheads mark LSR-LSR co-localization. Scale bar: 5 μm. |
