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FIGURE 5
Analysis of ciliation in adult Xenopus tissues. (a–d) Confocal micrographs of immunostained adult Xenopus tissues. (a–d) Micrographs of (a,b) adult stomach samples, (c) the adult trachea, and (d) the adult lung stained for cilia (Ac.‐α‐tubulin, cyan), mucins (PNA, magenta), and F‐actin (actin, green) reveal lack of multiciliated cells (MCCs) in these tissues. (e) Micrographs of adult oviduct sample stained for cilia (Ac.‐α‐tubulin, magenta) and mucins (UEA, green) reveal a densely ciliated epithelium populated predominantly by MCCs with mucus secretory cells being interspersed. Images in (c–e) were reconstructed from multiple individual images |
