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Fig. 4. Development of cranial and lateral line nerves. Acetylated tubulin immunostaining of cranial nerves in a control embryo (A) and in embryos in which the ear was transplanted (B,C). (D) Acetylated tubulin (green) immunostaining of lateral line nerves in the cranial skin of a control embryo (E) Acetylated tubulin (green) and Hoechst (blue) immunostaining of a later-stage transplanted embryo. Acetylated tubulin (green) immunostaining of lateral line nerves in the cranial skin of a control embryo (F) and an embryo in which the ear was transplanted (G). (H) Higher magnification of boxed area in E. Areas where native ears are present and where ablated ears had occupied are circled with solid and dotted lines, respec- tively. Native ears are labeled ar Eyes (A,B) and skin over the eyes (D,E,F,G) are labeled ye Cranial nerves are labeled with Roman Numerals. LL, lateral line; U, utricle; S, saccule; L, lagena; Ac, anterior canal; Hc, horizontal canal; Pc, posterior canal. Scale bar is 250 m. |
