| |
Figure 3. Regulation of Xenopus R-spondins by Wnt Signaling(A) Comparison of XRspo2, XWnt8, and XWnt3A expression pattern in early neurula embryos by whole-mount in situ hybridization. Dorsal view, anterior up.(B) Top: Diagram of experiment. 4-cell stage embryos were injected with 50 pg pCS-ppl (preprolactin), pCS-XWnt8, or pCS-β-catenin into each blastomere, and DMZs were dissected, cultured until stage 11 equivalent, and analyzed by RT-PCR. Bottom: RT-PCR analysis of the indicated genes. −RT, minus reverse transcription control.(C–E) 4-cell stage embryos were injected with 50 pg pCS-Wnt8, pCS-Wnt3A, or pCS-ppl into one blastomere and fixed at stage 11 for in situ hybridization with XRspo2. |
