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Fig. 3. Expression of wnt5a and wif1 in the mesenchyme of Xenopus lung. (A) WISH image shows wnt5a expression in the lung bud at stage 39. (B) Transverse section of a stage 39 embryo depicts wnt5a expression in the lung mesenchyme. (B′) DAPI stained section for the boxed region in (B) to better depict lung structure (outlined). (C) Stage 41 WISH shows wnt5a expression in the lungs. (D and E) Expression of wnt5a in the lung mesenchyme revealed by transverse section shows high expression in the lateral lung tubes. (E′) The magnified image of the boxed region in (E) indicates that wnt5b expression is restricted to the mesenchyme. (F) wif1 is expressed in the lung bud at stage 39. (G) Transverse section confirms presence of wif1 expression in the lung mesenchyme. (G′) DAPI stained section in the boxed region in (G) to better depict lung structure (outlined). (H) wif1 expression in a stage 41 embryo lung is shown in a dorsal view. (I and J) Transverse section of a stage 41 embryo illustrates wif1 expression in the anterior and posterior lung mesenchyme. (J′) Merged bright field and DAPI image of the boxed region in (J) shows wif1 expression in the mesenchyme. Red arrows indicate lungs. Abbreviations: es, esophagus; nc, notochord; ep: epithelium; mc: mesenchyme. |
