XB-CLONE-2046366
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Clone Name: spry2-723
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Gene:
spry2.S
,
spry2.L
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Species: Xenopus laevis
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Description: EXRC Clone Number 723. Xenopus laevis Sprouty homolog 2. Image EST 5513422. Full length cDNA, Accession BC073605. To generate probe for in situ.
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Sequence:
Full length sequence: BC073605 
[+]
GAGAGCGGAGCAGCTCAGTCACCTCGCTAGTATCCGGCAGAGTGTAAGCTCCGCTGTCACACGCACTAAAGGCAGCACTTTTCCCTGCACATACACCTCT
GGGATTTCTCTCTACAAGTTGCCTTCACTTGCTGCAACACTTTAGCAGTTGTATCGGATTGTACTCGCACTTTATTTAGCGGATACAAGGATCGCGCTGG
AGGAGACTGGAGTCCTGTGTCATGTTTTCTCTGTCCCTTCCTATTCGGGGAGCGCTGGCTGAAAGTTGCCCGGAGTGAAGCCCAGGGATAATCTCCCTGT
TACCCTGTAGGGTCCCGGCCGGCGGGTAGAGGAGGTCACGCAGGATTTCAGATGTGTTCTCAGCCCACTGGTGTGAAAACTCTTCAGAGTACTGATGGAG
ACGAGAGTACAAAATGGCAACGTATCCCAGCCTTTGCTCCCGGCTCGGCGTGAGACTGACCCACGGGAAATCCTGACGCAACAAGTTCACGTTTTGTCTT
TAGAACAAATAAGAGCCATAAGAAACACTAATGAATATACAGAAGGGCCCACAGTGGCTCCTCGCCCTGTGATTAAATCTGCTCCACGACCGACTACCCA
ACAGAAAAATGAAAGAATACACGTGGTAAATGAGCAGCAACCTTTTGTCAGGAGTCCACATCCACCAATGCATTCCTTGTCTCAGGCACCTTTGTCTCGA
TCAGTTGGTACAGTCGTTTCATCTTCTCGGAGCAATACAAGGACAAATAGCAGTTCTTCAGAGCAAAGGCTTCTTGCGCCACCATTTACATCTTCAGGAT
TGGTTGCAGACCGAATAATTAGAGTACAGCCCAAACCTGAGCTGAAATCTGACATTTTAAAGCCCTCTAGTAAAGAGGAATTGGGCTTACATTCATTAAG
GTGTGAGGATTGTGGGAAATGTAAGTGCCAAGAATGCACTTATCCAAGGACTCTCCCATCTTGTTGGATTTGTGACAAGCAGTGCCTTTGCTCAGCCCAG
GAAGTAGTTGACTATGGAACTTGTGCTTGTTGTGTGAAGTGCCTTTTCTATCACTGTTCAAATGATGACGAGGATAATTGTGCAGACAACCCATGTTCAT
GCAGTCAGTCCCACTGCTGCTCTCGATGGTCTGCTATCGGTGTCATGGCCTTGTTCTTGCCGTGTTTGTGGTGTTACCTTCCAGCCAAGGGTTGCCTTAA
ATTGTGCCAGGGCTGTTACGATCGTGTCAATAGACCTGGATGTCGATGCAAAAGATCAAACACAGTCTGCCTTAAAGTTCCACACCTTCAGCCCCGGAAC
TTAGAAAAACCAACATAGCACAGGTGAATGGATGTTTAAAAAGAACATAATAACTTCCTTTTAACACTCATGCAACCATCTTGTTACATTTAGGAAAAAG
CTTTGGGATAGGATTACCTGTTTGCGGTGGAATACTTATGCGTCAGCGGATTTAGCCTTAATACAGACCCTCTAATAAAGCTTGCAGTAGGATTGTGTTC
TGTGACAATTATTGTGTAAGCTATCACAGCACAGACACTCCTATGTACAATTGTATTCTTTGTGAATAGTACTTGGAACATTTGTAAAATTTACAAATGG
CTTTTTTTTTTTTTGCCAGAGATATTATGTTATATTTTTGTACATAAACCATATTTACAACTGTGAAAAAAGTGCCATTTTTTTATGGCACAGTCACAAA
ATTTACTAATGTACATTCAAAAGAATGTGAATCAATCAGTATGGTTAGATTGTCCTTCGCCTTCTGGAACTTTAATATTACCAGACTTCTATTCTTTGGA
TCTTATGTATATTGTACAGTTACACTGTAAGATTCAGCATTTATTTTCTAATGTTTCAATATTGTTTAGTGTAAAGTATATTTATTTGAAGTTTTATTAT
TTTATAAAGAGAAATATTTATTTTATGAAGCATCTTACAAAAGTCTCCCCTTTTTATGATGTCAGTTTCTACAAATCGGGGGTGACTGAGACATATGTTC
ACTATAAAATAGAAAATATATTAACGTCTGAAAATGAAAAAAAAAAAAAAA
5' EST: CF271833 [+]cccacgcgtccgcccattcgtccgcccgagagcggagcagctcagtcacctcgctagtatccggcagagtgtaagctccgctgtcacacgcactaaaggc
agcacttttccctgcacatacacctctgggatttctctctacaagttgccttcacttgctgcaacactttagcagttgtatcggattgtactcgcacttt
atttagcggatacaaggatcgcgctggaggagactggagtcctgtgtcatgttttctctgtcccttcctattcggggagcgctggctgaaagttgcccgg
agtgaagcccagggataatctccctgttaccctgtagggtcccggccggcgggtagaggaggtcacgcaggatttcagatgtgttctcagcccactggtg
tgaaaactcttcagagtactgatggagacgagagtacaaaatggcaacgtatcccagcctttgctcccggctcggcgtgagactgacccacgggaaatcc
tgacgcaacaagttcacgttttgtctttagaacaaataagagccataagaaacactaatgaatatacagaagggcccacagtggctcctcgccctgtgat
taaatctgctccacgaccgactacccaacagaaaaatgaaagaatacacgtggtaaatgagcagcaaccttttgtcaggagtccacatccaccaatgcat
tccttgtctcn
Library:
Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.4 kb. Constructed by Life Technologies. Note: this is a Xenopus Gene Collection library.| Anatomy: spleen | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
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Whole mount in situ hybridization:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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T7 |
SalI |
SmaI or EcoRI can also be used. |
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Suppliers:
Search for clone at:
EXRC
