XB-CLONE-3648186
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Clone Name: IMAGE:7655835
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Gene:
pou3f4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127328 
[+]
GGGATCCGCAGCAAGCATGGCCACGGCTGCCTCTAATCCCTACAGCATTCTCAGCTCCAGCTCCCTGGTCCATGACTCTGCGATCATGCAGCAAGGCAGC
CCTTTCAGGAATCCGCAAAAACTACTCCAAAGTGACTACCTGCAGGGAGTTCCCAGCAACGGGCATCCTCTCGGGCACCACTGGGTGACCAGCTTGAGTG
ATGCCAATCCCTGGTCTACCAGCCTAGGCAGCAGCCCATTGGACCAACAGGATATTAAACCCGGGAGAGAGGACCTTCAACTGGGGGCCATCATCCACCA
CAGGTCACCCCATGTCACCCATCACTCGCCTCATACCAATCACCCAAACGCCTGGGGGGCCAGCCCGGCTCATAACTCCTCGCTAACCTCCAATGGGCAA
CCTATCAATGTCTACTCCCAGTCCGGCTTTACGGTCAGTGGCATGTTGGACCATGGGGGGCTGACACCACCCCCACCATCAGGCACCACGCAGAGCCTGC
ACCCTGTGCTGAGAGAGCCCGTCGACCATGGGGACCTGGGCTCCCATCACTGCCAGGACCATTCGGACGAGGAAACCCCGACATCGGACGAGCTGGAACA
GTTTGCAAAGCAGTTCAAACAAAGGCGGATCAAGCTGGGCTTCACCCAGGCAGATGTTGGCTTGGCTCTGGGGACACTCTATGGCAATGTTTTCTCCCAA
GCCACCATCTGCAGGTTTGAAGCCTTGCAGCTCAGTTTTAAGAACATGTGCAAACTGAAACCTCTTCTAAACAAGTGGCTGGAAGAGGCAGACTCCTCCA
CAGGTAGCCCCACCAGCATTGATAAGATAGCAGCCCAGGGTAGGAAAAGGAAGAAGAGGACCTCTATTGAGGTGAGTGTGAAAGGAGTGTTGGAAACCCA
TTTCCTCAAGTGTCCCAAACCCGCAGCTCAGGAGATTTCCTCCTTGGCAGACAGCCTGCAGCTAGAGAAAGAAGTGGTCCGGGTTTGGTTTTGTAACAGA
AGGCAAAAAGAGAAAAGAATGACTCCTCCAGGAGACCCCCAGCAACACGAAGTCTATTCTCACAGTGTGAAAACAGACACCTCCTGCAATGAGCTTTGAC
TGAGGCAAGGCTCACATAAGTCGGGACTGAAGGAAACTGGATTTTTAAAGGGTTTCTAACTTATATTTTGGCGGAACAAAATACAAAAAATGTGACTTAG
GAGCTAAACGTTCCAAAAATATCCTGGGCAATATATAACTGACAATTACATACTGTTTACTCTAAACAAGGTTCGTTCTTTTTGGTGCCCTACCAGAAAG
GAGACATGCGTGCCTCTGATTAACCTCCCAGCGCCTTGGTTCCATCAGCTAAATTTCAGGTGATTTTGTTTCACAATAGAGTAGATTTATTTGCATATGG
AGATTATCTGGGATTTTTTTTTAATGTCTAAGGTGTTTTACTACAGCCTGTACACAATATTTGTGACAAAAATACATTGTGTTCTCCAATCAGCCCTATG
GATAATCATCATTCCTCATTTTATTTGATTTTTTTTTCTGCTGTTTTGTTTTTTTTGCAGTGTTAGAAGGTGTACATGGATATATCTGTAACTAGTTACC
AGAACCATGGAGCGTATATATTCATATAAATCCTTATTTAACCAACTGGTGGATATAATCATTTCTGCATGTCATTTATTGGACAGTTATTTAAGACTTT
CCAGTCTTACCATATCATTATGCATTTATATATGTCTTTTTTGCCTCTTGCTTTTTCTTCTTCTTTTAATAAACTGGCACTTTCCAAAAAAAAGGATAGA
AAATAGACAAAGAAACATGCAGATGAACATGTATTGCAGTAGCTTTTGATGACAAGTAATAATGTGGTATGTTATTACCTGCTAAGGGTTAGTAGGGCTT
AGGGATGGTGGAAGGTACTGTATGTAGTAAAACACCAGTCTATATGGTAAATCAATCTCCACTTTGAGGTTTGGCAAATGTGCTCGCTTCATCATCAGCC
TGTAGATTGGAGACATCCATTTCATTTTCTGTGCATTGTGTTCACTGTGAGCAGCCAGAGGGGATCTAAGCTGAGCCTGACAGGCACCACTGATGCTGTG
TCCCTCAGTGAGCGACAACTGTGAGGGGAAGGCTTGCACTTCACAGGTCAGAGATTTGCACTAAACATATACTCCTAGCTATATTATCATAACAATGGCT
TCTATATTTGTATTTCAGCCACTTTTACCATCTTTTATTTTTTGTTGTTGTCAGGCAGAACATTAGCTTTCTTGCTTTATGGGGTGTCTAGCAAAGTATC
ATGCATTTCTATTCTATGCATAGAATAGAAATTATTTGTATACTGGGGCCTAATTTTTACTCCAGCACAGAGCTATTACCTATATACAGACACACTTTAT
AAAACATAATAACACTGGGGCTTCTTTCAGTGCTACTTTTATGTAGTAATAGTCAATCAGATACACACATTTCCCTGTGTGTGTATATACATATACATAT
ATATATATACACACACACAGCCACATACACATACACATACACACAGCCTTTCATATCTGATTCTACTGTTTAAAGTTGAGATCCCAGGCTACCCTTACAT
ACTCTGGCCCTGCAGTTCCAGAAAGCAGAATGCACTTCCACCTAATTCCACCATTTCTTTACCTTCCCATATTCAAGTCTTCTCTATTTGGAGCTATAGG
ACTAGTTCTAGATCGCGA
5' EST: CX826148 [+]ggatccgcagcaagcatggccacggctgcctctaatccctacagcattctcagctccagctccctggtccatgactctgcgatcatgcagcaaggcagcc
ctttcaggaatccgcaaaaactactccaaagtgactacctgcagggagttcccagcaacgggcatcctctcgggcaccactgggtgaccagcttgagtga
tgccaatccctggtctaccagcctaggcagcagcccattggaccaacaggatattaaacccgggagagaggaccttcaactgggggccatcatccaccac
aggtcaccccatgtcacccatcactcgcctcataccaatcacccaaacgcctggggggccagcccggctcataactcctcgctaacctccaatgggcaac
ctatcaatgtctactcccagtccggctttacggtcagtggcatgttggaccatggggggctgacaccacccccaccatcaggcaccacgcagagcctgca
ccctgtgctgagagagcccgtcgaccatggggacctgggctcccatcactgccaggaccattcggacgaggaaaccccgacatcggacgagctggaacag
tttgcaaagcagttcaaacaaaggcggatcaagctgggcttcacccaggcagatgttggcttggctctggggacactctatggcaatgttttctcccaag
ccaccatctgcaggtttgaagccttgcagctcagttttaagaacatgtgcaaactgaaacctcttctaaacaagtggctggaagaggcagactcctccca
cagtagccccaccagcattgat
3' EST: CX826147 [+]tcgcgatctagaactagtcctatagctccaaatagagaagacttgaatatgggaaggtaaagaaatggtggaattaggtggaagtgcattctgctttctg
gaactgcagggccagagtatgtaagggtagcctgggatctcaactttaaacagtagaatcagatatgaaaggctgtgtgtatgtgtatgtgtatgtggct
gtgtgtgtgtatatatatatatgtatatgtatatacacacacagggaaatgtgtgtatctgattgactattactacataaaagtagcactgaaagaagcc
ccagtgttattatgttttataaagtgtgtctgtatataggtaatagctctgtgctggagtaaaaattaggccccagtatacaaataatttctattctatg
catagaatagaaatgcatgatactttgctagacaccccataaagcaagaaagctaatgttctgcctgacaacaacaaaaaataaaagatggtaaaagtgg
ctgaaatacaaatatagaagccattgttatgataatatagctaggagtatatgtttagtgcaaatctctgacctgtgaagtgcaagccttcccctcacag
ttgtcgctcactgagggacacagcatcagtggtgcctgtcaggctcagcttagatcccctctggctgctcacagtgaacacaatgcacagaanatgaaat
ggatgtctccaatctacaggctgatgatgaagcgagcacatttgccaaacctcnaagtggagattgatttaccatatagactggtgttttactacataca
gtaccttccaccatccctaagccctactaacccttagca
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
