XB-CLONE-3650873
|
Clone Name: IMAGE:7653992
|
Gene:
cbarp
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
Full length sequence: BC135992 
[+]
GGTGTTGAGAAGCGGGCACCGGGCATAGTACCATCTGGGGAAGGAGACGGGCTGGGAAACAGCTGGGCCGGGAGAGAAAGTGTAAGATCATGAGCTATGA
GTCACCTGCATGGAACAATATAAATGATAGCTCCACGACCACTCCCAGTACACCCTCACAAGATGGGTATGTTTTACTCTTGGTTCTGCTCTCAATCTTT
ATCGGAGGAACACTTCTTCTACTCACTGGGGTCCTTATTCTCTGCAAACGCTGCTGTGACTCCCGTCTACGGCAATCTAGACCCAGTGATGATGCAGAAA
AAACAAACACTTCATATGTGGATGAATCCCAGCCACCTCATGAAATTACAATCCGAGTGGAGGATGCAGAGTCTTTGTCAGCCACAGCTCTCAGAGATAT
GGAGTCAGAGAGATTTTTGTGTACAACTTCCAGTGGAAGAAGAGTATCGTTTAATGAAGGTGCTCTTTGTGGACAAGAAAGGAGGGATAGGGAAAAAGGA
AGAAGGTACACCCTAACTGAAGGAGATTTTCACTATCTCAAAAATGCACGGCTTACATTTCAACCTCTCCCATCACTGCCCCCCACTGCTCTACACATCC
TAACCATACATGAGAGTGAAGGTGGTGAAAGCAGTAGCAGTGGAGGGGCTTCTGAGACAAGACCAACTTCTCCCAGCAAATCCAATATCTCAATATATCA
GCCCCCCCGCAGTTCTCCCGCACCAGTGACTCGCCTATCACTCAGTCTAAATTCTGCTCTTCCTGGAGATATGTACAATTCTGTGGCCGATGCCAGCTTT
ATGGAAACCCCAGTGCACAGTCCTCCCACCCCTAGACAAAGTCCAGCCAGTGACAATTTCGACTTCAACAGCCATCCCCAAGGTTGTAGAGGACCAGATG
TAGGGCCTAGTAGAGGACATGGTGCTGTCCTGCATTTTTTCAGCCGTCTGCGTCGCCATGCAAGTTTGGAAGGAGCAAGCCCTTATTTAAAGATCAAAAA
ATGGAAACTAGGAAATGTGCAGAGAGCAAGCAGCCTTGACACTAGAGGGTCTCCAAAGCGGCACCAGTTTCAACGTCAGAGAGCAGCAAGTGAGAGTGCT
GAACGAGATGATGTTATACAGTACATTGTTCGCACACAAGATACCTTTCCATCTTCCCCTGGACACTTCATCTCACAGCATGGGACCCCACCACACTCTC
TCGGCAGGTTAGACCATGGGGCAGAGGGCTCTCTATCAAATATAGGGAGTACCGAGCAGGCGCAGCTTCCCCCTCAGTGTGACATTTGGAGCTTACGTGC
ATCTCTGGAGCTTTGTGCTTCTGACCAAAGCAGCAGTAACAATGACCGTGACTCAGTTAGAAGTGATGCAGATAGTGTGAGCTCTTTGAGTGGACTTCCT
AGTCTTTCTTCCCAGGACCTGCCTGATGGCCGATCCACACGTGGTTCAGAGTCAGATACAGGATCCCGAAAACTACTACAGATGGACAGTGGTTATGCTT
CTATTGAAGCCCCCTGTCGCTCTGCAGAGGAGCCATGTAGCAGTCCTCGTGACAAAACTGCATCTGAGAAAAGACTGTTTTTCACATATGCTGGGAGAAA
AGGTACTGTCTTTGAGAGTTTGGAAGGTCGTTTATATGAACAAAGTGCTTCAAAAGGTGAGGAAGAAGCTTGGTATCCACAAGGCCAGGCACCAACCCCA
CGGGAATCCATCTCACGACGAGATTACAGCATTGATGAGAAAACAGATGCACTATTTAATGAGTTTCTAAGGCATGACCCCCAATATGATGACTCTCCTC
TGCGTATGAAGCATCGATCTCGTGCCCATCTTCGAAAACAGTGGCAGAGGACAAAGCAGTATAGTGACCCAGGAGTACGTTATCCCCCAGCACTGGACAG
ACACCGTGCACAACCACTACGTAGAGGAGACAGTACTAGCTACCTGACTGACACACGATACCACAGCACTTTGCCAAGGATTGCAAGCACCACAGATGAA
GAAGGGGTTGATGGCTGTCTGCAAAGCTCTGCTTCACTTACTCCTGAAGACAAAATCCAGGCAATCGAGGAGGAGCCCAGTGAGCACGGTCCAGGTCTTG
AAGAGCCCCGTCCTGCCCCAGATGATCCTGATCAAGATTATGGTTATGGACCACAAACAACAGAGCTACTTGAAAAGATAGGTGCAGGTTTAGAGGAGCG
GCTATACCCCAGTGTACAGAGGACAGCACGTAGTCCTGAAAGACTTTGCACTGTGGCATATATTTCACCAGATCATAGTCCAGTGTAGAACTGATGAAAA
GAGAGAAGAACCCAGGAATTTGCAGCAGGGAGGTAGATATTTTGTTACTGAACTAGTACAACATTCACTGCTTCCAAACACTGCAGTGCCAAATAATGGA
AAACAGTAGTAGCTCACTCATCCATTGTGTGTGTTTGTAATATATATTATAAATATATATATATACATACACACCTACATACATAGACATTCCCATCCTC
TCTCTCTCTCTCTCAAGCTGCCATACTGTGCTATATGGACACTAAGCATGACTGTAAATACAAGCAGCACATTTGCAACATGAACAAAACATCCCTAAGA
ACACATACAGCATGATGGCTCAAATTGCATCATTTACACAGGCACACAGAAGCTGTACAATTTTCTTCCAAGCAATATAAAATTTCTTCCTGACTACTGG
AAAATATGCACTGCCCTCAGGAATTCTGCACCAGGAAAGTATAGCAGGAAGGTATAAAGGCTCCTCCAGCTCTCTTTTTGTTTGCTGCCATTCTGGATCT
CTATATCCAAAAAAAAAAAAAAAAAAAA
5' EST: CX823143 [+]gtgttgagaagcgggcaccgggcatagtaccatctggggaaggagacgggctgggaaacagctgggccgggagagaaagtgtaagatcatgagctatgag
tcacctgcatggaacaatataaatgatagctccacgaccactcccagtacaccctcacaagatgggtatgttttactcttggttctgctctcaatcttta
tcggaggaacacttcttctactcactggggtccttattctctgcaaacgctgctgtgactcccgtctacggcaatctagacccagtgatgatgcagaaaa
aacaaacacttcatatgtggatgaatcccagccacctcatgaaattacaatccgagtggaggatgcagagtctttgtcagccacagctctcagagatatg
gagtcagagagatttttgtgtacaacttccagtggaagaagagtatcgtttaatgaaggtgctctttgtggacaagaaaggagggatagggaaaaaggaa
gaaggtacaccctaactgaaggagattttcactatctcaaaaatgcacggcttacatttcaacctctcccatcactgccccccactgctctacacatcct
aaccatacatgagagtgaaggtggtgaaagcagtagcagtggaggggcttctgagacaagaccaacttctcccagcaaatccaatatctcaatatatcag
cccccccgcagttctcccgcaccagtgactcgcctatcactcagtctaaattctgctcttcctggagatatgtacaattctgtggccgatgccagcttta
tggaaaccccagtgcacagtcctcccacccctagacaaagtccagccagtgacaatttcgacttcaacagccatcccc
3' EST: CX823142 [+]ggatatagagatccagaatggcagcaaacaaaaagagagctggaggagcctttataccttcctgctatactttcctggtgcagaattcctgagggcagtg
catattttccagtagtcaggaagaaattttatattgcttggaagaaaattgtacagcttctgtgtgcctgtgtaaatgatgcaatttgagccatcatgct
gtatgtgttcttagggatgttttgttcatgttgcaaatgtgctgcttgtatttacagtcatgcttagtgtccatatagcacagtatggcagcttgagaga
gagagagagaggatgggaatgtctatgtatgtaggtgtgtatgtatatatatatatttataatatatattacaaacacacacaatggatgagtgagctac
tactgttttccattatttggcactgcagtgtttggaagcagtgaatgttgtactagttcagtaacaaaatatctacctccctgctgcaaattcctgggtt
cttctctcttttcatcagttctacactggactatgatctggtgaaatatatgccacagtgcaaagtctttcaggactacgtgctgtcctctgtacactgg
ggtatagccgctcctctaaacctgcacctatcttttcaagtagctctgttgtttgtggtccataaccataatcttgatcaggatcatctggggcaggacg
gggctcttcaagacctggaccgtgctcactgggctcctcctcgattgcctggattttgtcttcaggagta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
