XB-CLONE-14574240
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Clone Name: isl1-SPH588
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Gene:
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Species: Xenopus laevis
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Description: EXRC Clone Number SPH-588.
Xenopus laevis ISL LIM homeobox 1.
To generate probe for in situ and mRNA for injection.
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Sequence:
Full length sequence: pBSxislet1_FullLength
[+]
NNNNNNNNNNGNCNNNNCGAGTTTTTTTTTTTTTTTTTTTTATCGATATTTTTTACTTCAGCAAACAAAAAATATAGTCAAATAAACATGAAAAGTGGCA
AGTCTTCCGACAATAAATAATAAATTACTTTATAATTTGCAATTCAAGAGCAAACAAAAAAAAAAAATGCTGCCTTTTTTTTCTCTTTATTTCCAGAGAG
GAACAGTCGTTTGAACCGATAACTATACACATCTTTGGGGGCAACAAAAAAAATCAAAGTCCTTGGACAGACAAGAATCAGACGCAATCCAGAGACGCAT
GTGGCAGAGAGAGAGAGAGGTATACCTTGTTGTTGCGATGCTTCCTAGACTTGGTTTGAGCATTAAAAAAAAAAAAAAATTATATTGGAAAAAAAAATAA
GCAGTGTCTTTAGCTGTTGTCTTTGTTTTACTCCCCCCCCCCCCATTAATGTAGTTTTTTTTAACGTTAATAAGGTTTCATGAGAAAGGACAAGTGGTGC
ATGCTATAGGCGACCTGGATTTCCACACCCCCAGGTGAAACACTACATGGCAACTCCAACATCTCACCCTGCAGGGATCCCAAGCACTGTCCCTTTAACT
TGAGTCTGGGGTGCTGCCTGCAGATCGATCGCAGCTCCTGTTTGGCAGGGCAGTGATTAAGTGATGTCAGGAGAGATAAAAGAAGCACCATTGGGATTAG
GGTGATCCCTCCTATCTGGGTCCATCTTGGTCTGTTCTGGGATGTTCCAATGGGGGGTATGGGGGGAAGGTTAAATGCTATTTTTGGAGATCCATAGATA
GAAATCCTCTACAGCACTCAATCTTGTCTGCCGTGATACTCCAACCACCATGTCCTTGTCCTTCACTCCTAGTAAGATCCCATGATTGTCTTCACAGTGT
GGCTGCCATCCCATGTTCTGTAGGCGGCCCCGCATCCTATGGAGTTGGCTGGATTCTCTGTCTGCTGGGTCCTAAAACACTTCCTTTCAGTCCAAGCTTT
GCCCTCAACNGAGGGNACCAGTCCATGNCCATCATGCCTCTATANGGCTNGCTACCATGCTGNTTGGGGTANNNGGNANCTGGNNNNANNNNNNNNCCTC
NNNNCCNNNNNANTNNANNNGNCTCNNNTGAAANNCNNNNANNANGNCANTGCTNNNNNNTGATNANGNNNNNNNTNNGNNANTNNNNNAGNNNNNNGGN
GNNNGNNNNANNNNCNNNNCNNNNNNNCNNNNNCNCCNNNNNNNTTTNGGNNCNNNNNN
Construction:
Insert:
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| Size: | Source Tissue: | Stage: |
Vector:| Vector Details | Vector Map |
Name: pBluescript II SK+
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
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RNA injection:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| sense |
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T3 |
XhoI |
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Whole mount in situ hybridization:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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T7 |
EcoRI |
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Suppliers:
Search for clone at:
EXRC
