XB-CLONE-3916569
Clone Name: IMAGE:7091247
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Gene:
msx1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653590 [+]
taattaatcccccccccccccgagtaaagtgaccaggtgccccgggctcagttgtatggatcgcactcgcccgctgtaacttcccagctcggatatctct
gtatggccccggctctgcttatggctacttaccagcccggggtgaaagtagaagaaagtccttctgtgaataagatgcaaaccggcctgaaggtggccct
gggcgaggacaagcccaaagtgcccgggatcctgcccttcagtgtggaggccctgatggccgataggaagcctgggagagaaagagacctgtccagcccc
actggatctccgctggcagggacttctcacagccccagggtgggctctttagcgcccggggagacccccaactcccccatatccattggaaaccgcttcc
ctgtggggggcatcatgaaactgccggaggaggcgctggtcaagccggagagcccagagaggagctcatggatccagagccccaggttctccccttcccc
aacaagtaagtgtccctctgtccttattgctgatctattgggggcacagcttgctcttctgggctgtagggctgatccctagggctctattgggggcaca
gcttgctcttctgggctgtagggctgatccctagggctctattgggggcacagcttgctcttctgggctgtaaggctgatccctaggctctattggggca
cagcttgctcttctggctgtaggctgatcctagggt
3' EST: CN320786 [+]tttttttttttttttgcattaaaattaatttatttaacaaatataactataatataaatgataataaatttcaaatatacaatatattacatattacata
caatcccttccaaagggattattgcaataacgaaacgcaaacagacagtgctgttaagtgaaagtttcaatactatattacatgtatctcttatatgtaa
aaaatattttactccctttatcaatcttgtcattctgtggaggtgccaagctccccaaacctaaagttacacagcaacaggtcccaccccaaattaagct
atttatccttaccccagtttgggtcaggggactactgatcccttctgcagcaaatccattaagctcccaatcctgcttgtaccatattataatatattta
cactgctcaccacctttgctgggaagcctttccatgcaatctgggataactcctgaagcagtggatgctttcaatgttacagatattccctccgttctgg
gatagcactaaggctgcccaattagggcttgggaagctggttgacacctgaaactcatggactactaattagacacagctaacaagatgacaacaatagg
catattgaagtgacatagtatttctacaattcaattccctaggaactcataaatagtgaaaaaatataggggtgcacagaaaattcaactcagtcttctt
gcccatagtccccatggagtgcgaccaagtcttangagagatgatacatgctgtatccnacattgggctgttgtaagtcccaaagagaaaaccgggcgaa
gccggcccccggggaaaagggtttgaagtccccaacaaagggggggggtttggggtactgagat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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