XB-CLONE-4350608
Clone Name: IMAGE:7025919
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Gene:
sgk2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785419 [+]
taattaatccccccccccccctagcaactcaaaaacaagattttagaattaagcagagggagggtgaaggcctccatttttttcaccgttatgtcctcaa
caataaacttggggccgtctgcaaatccaaatgccaaacctactgatttcgatttcttgaaagtgattggcaaaggaagctttggaaaggtccttctcgc
caagcgtaaatgtgacaataacttctacgcagtgaaggtcctgcagtaaaagaccattctcaagaaaaaggagcaaaacgcacatcttggcccagaagga
acgtccgtgctggaaaaaaccctctagccatccaatttctgggtggggggctggcactaactttcctttccaaaattgtccgaaaaaaaactccttaact
ttgggaaaaggggaaattatgtttccaaattgggaaagggggaaatctttaatttcctttaagcgtttgttaacaaaaggggggaaaaaaaaaggttttt
tcccccaaaaaaaaaaaccccctgtggggccccaccctaaatttttttaatttcgagcaaacacacaaaataaaaatgatgggccgctctgtattggggc
gtctcccctgttttggggggggattatttaaactcaataatccaacaagaaaatactttcctcccgctaagaaagaaaaactcacgcacactttattttc
tttctcacccaatagagaaaaatatttgtttttacaactccccccctgtgggagagaaataataataccctccctgcagctgttggggagagaaaaaaac
aaaacacacagggggcgtttttattaggatggggggtgggggcgcctctacgcataaaaaataatttaataaggccgattatattttctttacatacaaa
aataataccaactacttatatatactccctccggggttgagtatgaaacccccccccgagagtaatctcctctttcgtttcggtgngaacaacccccccc
atggtaa
3' EST: CF785418 [+]tttttttttttttttcgtttgtaccaatcagctttatttattcttcatgtcaaatacaatttattatctgtatcacatttttataaaaatatagtccttg
gtttcaggcacaatatgcgcagaccttgcctccaaaaatgaaacggaatgttgaaccaggaggaaaatatttatttcgtgaaaatcgacgtttttgtgtt
gcagctgagcgggtcgtgatgggataaatgaagtgttttggtagaaaaaggacagaactgagtaagtgatacagtgggttatgggtaacgtcgctgagtt
tgcctaaggatccagttaggcagatatcatagagctgaatgccccctgcataccatggttgctattgtaaggggcaactccggcttcagaacctaaatta
gtggggaggaacagggggcggggcttagggaactgttccaaaccatattattacattaaaaatcattaataggctgcatttttttaaactgatgtatatt
gcaaagttgtttaaaattatgtttacttttcttaaagcttaagttgtttttaggtggagttaagtaggctatcagtaactggctgctgtgaactgaatca
caagcagccaatagcttatctaattatgtgccctcttgggcctataatctgaaaagagcagtgtccgactggcccatcagggatacccaaaaaactccct
aatgggccccaggtggtcagtggggccccttcctgcctgatcatttgtcccggcttcgtgggtcatttccctattttctgaaatcggaaaccaaattaaa
aa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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