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GEO Series: GSE76991

Title: Differential expression analysis of Gcn5 knockdown embryos at early developmental stage 10.5 in Xenopus laevis

Summary

We sequenced cDNA prepared from ribosomal RNA depleted total RNA of 10-10 embryos injected with Gcn5-antisense oligonucleotides and with water to create expression profiles of Gcn5 knockdown and control, respectively, in Xenopus laevis at early embryonic developmental stage 10.5. (according to Nieuwkoop and Faber), and we executed differential expression analysis of sequence count data (DEseq).


Contributors: Gert Veenstra, E Gazdag, I Kruijsbergen, G Veenstra

Experiment Type: Differential expression analysis using RNA sequencing

Phenotypes:
Xla Wt + kat2a KD + WE NF10-10.5 - RNA-Seq (RNA)

Article: XB-ART-51942, PubMed

Source: NCBI GEO, Xenbase FTP

Samples: (DEG = Differentially Expressed Genes; GSM = GEO Sample Number)
Sample View GSMs Assay Type
WE - NF10-10.5 GSM2042206  GSM2042207  RNA-Seq
Background: Xla.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10 to NF stage 10.5  
   WE + kat2a KD - NF10-10.5 DEG GSM2042204  GSM2042205  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
DMED injection kat2a.L NF stage 1 blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10 to NF stage 10.5  
Select All DEG      Select All 
Xtr   
Xla 

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