Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Neurosci
2000 Oct 15;2020:7517-24. doi: 10.1523/JNEUROSCI.20-20-07517.2000.
Show Gene links
Show Anatomy links
Mutants of a temperature-sensitive two-P domain potassium channel.
Kunkel MT
,
Johnstone DB
,
Thomas JH
,
Salkoff L
.
???displayArticle.abstract???
Within the Caenorhabditis elegans genome there exist at least 42 genes encoding TWK (two-P domain K(+)) channels, potassium channel subunits that contain two pore regions and four transmembrane domains. We now report the first functional characterization of a TWK channel from C. elegans. Although potassium channels have been reported to be activated by a variety of factors, TWK-18 currents increase dramatically with increases in temperature. Two mutant alleles of the twk-18 gene confer uncoordinated movement and paralysis in C. elegans. Expression of wild-type and mutant TWK-18 channels in Xenopus oocytes showed that mutant channels express much larger potassium currents than wild-type channels. Promoter-green fluorescent protein fusion experiments indicate that TWK-18 is expressed in body wall muscle. Our genetic and physiological data suggest that the movement defects observed in mutant twk-18 animals may be explained by an increased activity of the mutant TWK-18 channels.
Brenner,
The genetics of Caenorhabditis elegans.
1974, Pubmed
Brenner,
The genetics of Caenorhabditis elegans.
1974,
Pubmed
Caterina,
The capsaicin receptor: a heat-activated ion channel in the pain pathway.
1997,
Pubmed
,
Xenbase
Chavez,
TWIK-2, a new weak inward rectifying member of the tandem pore domain potassium channel family.
1999,
Pubmed
,
Xenbase
Davis,
A mutation in the C. elegans EXP-2 potassium channel that alters feeding behavior.
1999,
Pubmed
Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed
Duprat,
TASK, a human background K+ channel to sense external pH variations near physiological pH.
1997,
Pubmed
,
Xenbase
Elkes,
EGL-36 Shaw channels regulate C. elegans egg-laying muscle activity.
1997,
Pubmed
Fink,
A neuronal two P domain K+ channel stimulated by arachidonic acid and polyunsaturated fatty acids.
1998,
Pubmed
,
Xenbase
Heginbotham,
Mutations in the K+ channel signature sequence.
1994,
Pubmed
,
Xenbase
Hosono,
Temperature-sensitive mutations causing reversible paralysis in Caenorhabditis elegans.
1985,
Pubmed
Huang,
The lin-15 locus encodes two negative regulators of Caenorhabditis elegans vulval development.
1994,
Pubmed
Johnstone,
Behavioral defects in C. elegans egl-36 mutants result from potassium channels shifted in voltage-dependence of activation.
1997,
Pubmed
,
Xenbase
Kindler,
Local anesthetic inhibition of baseline potassium channels with two pore domains in tandem.
1999,
Pubmed
,
Xenbase
Leonoudakis,
An open rectifier potassium channel with two pore domains in tandem cloned from rat cerebellum.
1998,
Pubmed
,
Xenbase
Lesage,
TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with a novel structure.
1996,
Pubmed
,
Xenbase
Lesage,
Dimerization of TWIK-1 K+ channel subunits via a disulfide bridge.
1996,
Pubmed
,
Xenbase
Liu,
Gated access to the pore of a voltage-dependent K+ channel.
1997,
Pubmed
Loukin,
Random mutagenesis reveals a region important for gating of the yeast K+ channel Ykc1.
1997,
Pubmed
,
Xenbase
Maingret,
TRAAK is a mammalian neuronal mechano-gated K+ channel.
1999,
Pubmed
Mello,
Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.
1991,
Pubmed
Mendel,
Participation of the protein Go in multiple aspects of behavior in C. elegans.
1995,
Pubmed
Patel,
A mammalian two pore domain mechano-gated S-like K+ channel.
1998,
Pubmed
Patel,
Inhalational anesthetics activate two-pore-domain background K+ channels.
1999,
Pubmed
Perozo,
Structural rearrangements underlying K+-channel activation gating.
1999,
Pubmed
Reiner,
Analysis of dominant mutations affecting muscle excitation in Caenorhabditis elegans.
1995,
Pubmed
Reyes,
Cloning and expression of a novel pH-sensitive two pore domain K+ channel from human kidney.
1998,
Pubmed
Salinas,
Cloning of a new mouse two-P domain channel subunit and a human homologue with a unique pore structure.
1999,
Pubmed
,
Xenbase
Salkoff,
Surfing the DNA databases for K+ channels nets yet more diversity.
1995,
Pubmed
Soreq,
Xenopus oocyte microinjection: from gene to protein.
1992,
Pubmed
,
Xenbase
Thomas,
Genetic analysis of defecation in Caenorhabditis elegans.
1990,
Pubmed
Wei,
Eight potassium channel families revealed by the C. elegans genome project.
1996,
Pubmed
Wei,
Calcium sensitivity of BK-type KCa channels determined by a separable domain.
1994,
Pubmed
Weinshenker,
Block of an ether-a-go-go-like K(+) channel by imipramine rescues egl-2 excitation defects in Caenorhabditis elegans.
1999,
Pubmed
Williams,
Genes critical for muscle development and function in Caenorhabditis elegans identified through lethal mutations.
1994,
Pubmed
Zerangue,
A new ER trafficking signal regulates the subunit stoichiometry of plasma membrane K(ATP) channels.
1999,
Pubmed
,
Xenbase
