Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
2000 Mar 01;206:2031-42. doi: 10.1128/MCB.20.6.2031-2042.2000.
Show Gene links
Show Anatomy links
p300 requires its histone acetyltransferase activity and SRC-1 interaction domain to facilitate thyroid hormone receptor activation in chromatin.
Li J
,
O'Malley BW
,
Wong J
.
???displayArticle.abstract???
We have characterized the mechanism by which coactivator p300 facilitates transcriptional activation mediated by the heterodimer of thyroid hormone (T3) receptor and 9-cis retinoid acid receptor (TR-RXR) in the context of chromatin. We demonstrate that, while p300 can enhance the transcriptional activation mediated by both liganded TR-RXR and GAL4-VP16, its histone acetyltransferase activity (HAT) is required for its ability to facilitate liganded TR-RXR- but not GAL4-VP16-mediated transcriptional activation. To understand how p300 is recruited by liganded TR-RXR, we have analyzed the interactions between TR-RXR and p300 as well as SRC-1 family coactivators. We show that, in contrast to a strong hormone-dependent interaction between TR-RXR and SRC-1 family coactivators, p300 displays minimal, if any, T3-dependent interaction with TR-RXR. However, p300 can be recruited by liganded TR-RXR through its interaction with SRC-1 family coactivators. Consistent with the protein-protein interaction profile described above, we demonstrate that the SRC-1 interaction domain of p300 is important for its ability to facilitate transcriptional activation mediated by TR-RXR, whereas its nuclear receptor interaction domain is dispensable. Collectively, these results reveal the functional significance of the HAT activity of p300 and define an indirect mode for the action of p300 in TR-RXR activation.
Allfrey,
Histone acetylation in insect chromosomes.
1968, Pubmed
Allfrey,
Histone acetylation in insect chromosomes.
1968,
Pubmed
Almouzni,
Replication-coupled chromatin assembly is required for the repression of basal transcription in vivo.
1993,
Pubmed
,
Xenbase
Baniahmad,
Modular structure of a chicken lysozyme silencer: involvement of an unusual thyroid hormone receptor binding site.
1990,
Pubmed
Baniahmad,
Interaction of human thyroid hormone receptor beta with transcription factor TFIIB may mediate target gene derepression and activation by thyroid hormone.
1993,
Pubmed
Blanco,
The histone acetylase PCAF is a nuclear receptor coactivator.
1998,
Pubmed
Chakravarti,
Role of CBP/P300 in nuclear receptor signalling.
1996,
Pubmed
Chang,
A thyroid hormone receptor coactivator negatively regulated by the retinoblastoma protein.
1997,
Pubmed
Chen,
A transcriptional co-repressor that interacts with nuclear hormone receptors.
1995,
Pubmed
Chen,
Nuclear receptor coactivator ACTR is a novel histone acetyltransferase and forms a multimeric activation complex with P/CAF and CBP/p300.
1997,
Pubmed
Chen,
Regulation of hormone-induced histone hyperacetylation and gene activation via acetylation of an acetylase.
1999,
Pubmed
Dilworth,
Ligand-dependent activation of transcription in vitro by retinoic acid receptor alpha/retinoid X receptor alpha heterodimers that mimics transactivation by retinoids in vivo.
1999,
Pubmed
Eckner,
p300 and CBP as transcriptional regulators and targets of oncogenic events.
1996,
Pubmed
Evans,
The steroid and thyroid hormone receptor superfamily.
1988,
Pubmed
Fondell,
Unliganded thyroid hormone receptor alpha can target TATA-binding protein for transcriptional repression.
1996,
Pubmed
Fondell,
Ligand induction of a transcriptionally active thyroid hormone receptor coactivator complex.
1996,
Pubmed
Glass,
Positive and negative regulation of gene transcription by a retinoic acid-thyroid hormone receptor heterodimer.
1989,
Pubmed
Gregory,
Chromatin and transcription--how transcription factors battle with a repressive chromatin environment.
1998,
Pubmed
Gu,
A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation.
1999,
Pubmed
Gu,
Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain.
1997,
Pubmed
Hebbes,
A direct link between core histone acetylation and transcriptionally active chromatin.
1988,
Pubmed
Heinzel,
A complex containing N-CoR, mSin3 and histone deacetylase mediates transcriptional repression.
1997,
Pubmed
Hong,
GRIP1, a novel mouse protein that serves as a transcriptional coactivator in yeast for the hormone binding domains of steroid receptors.
1996,
Pubmed
Hörlein,
Ligand-independent repression by the thyroid hormone receptor mediated by a nuclear receptor co-repressor.
1995,
Pubmed
Ikeda,
Activation domain-specific and general transcription stimulation by native histone acetyltransferase complexes.
1999,
Pubmed
Kamei,
A CBP integrator complex mediates transcriptional activation and AP-1 inhibition by nuclear receptors.
1996,
Pubmed
Kawasaki,
Distinct roles of the co-activators p300 and CBP in retinoic-acid-induced F9-cell differentiation.
1998,
Pubmed
Kim,
Molecular cloning of xSRC-3, a novel transcription coactivator from Xenopus, that is related to AIB1, p/CIP, and TIF2.
1998,
Pubmed
,
Xenbase
Kliewer,
Retinoid X receptor interacts with nuclear receptors in retinoic acid, thyroid hormone and vitamin D3 signalling.
1992,
Pubmed
Korzus,
Transcription factor-specific requirements for coactivators and their acetyltransferase functions.
1998,
Pubmed
Kraus,
p300 and estrogen receptor cooperatively activate transcription via differential enhancement of initiation and reinitiation.
1998,
Pubmed
Lazar,
Differential DNA binding by monomeric, homodimeric, and potentially heteromeric forms of the thyroid hormone receptor.
1991,
Pubmed
Le Douarin,
Ligand-dependent interaction of nuclear receptors with potential transcriptional intermediary factors (mediators).
1996,
Pubmed
Li,
RAC3, a steroid/nuclear receptor-associated coactivator that is related to SRC-1 and TIF2.
1997,
Pubmed
McInerney,
Determinants of coactivator LXXLL motif specificity in nuclear receptor transcriptional activation.
1998,
Pubmed
Nagy,
Nuclear receptor repression mediated by a complex containing SMRT, mSin3A, and histone deacetylase.
1997,
Pubmed
Nawaz,
The Angelman syndrome-associated protein, E6-AP, is a coactivator for the nuclear hormone receptor superfamily.
1999,
Pubmed
Ogryzko,
The transcriptional coactivators p300 and CBP are histone acetyltransferases.
1996,
Pubmed
Ogryzko,
Histone-like TAFs within the PCAF histone acetylase complex.
1998,
Pubmed
Oñate,
Sequence and characterization of a coactivator for the steroid hormone receptor superfamily.
1995,
Pubmed
Puzianowska-Kuznicka,
Both thyroid hormone and 9-cis retinoic acid receptors are required to efficiently mediate the effects of thyroid hormone on embryonic development and specific gene regulation in Xenopus laevis.
1997,
Pubmed
,
Xenbase
Spencer,
Steroid receptor coactivator-1 is a histone acetyltransferase.
1997,
Pubmed
Struhl,
Chromatin structure and RNA polymerase II connection: implications for transcription.
1996,
Pubmed
Takeshita,
TRAM-1, A novel 160-kDa thyroid hormone receptor activator molecule, exhibits distinct properties from steroid receptor coactivator-1.
1997,
Pubmed
Torchia,
The transcriptional co-activator p/CIP binds CBP and mediates nuclear-receptor function.
1997,
Pubmed
Treuter,
Competition between thyroid hormone receptor-associated protein (TRAP) 220 and transcriptional intermediary factor (TIF) 2 for binding to nuclear receptors. Implications for the recruitment of TRAP and p160 coactivator complexes.
1999,
Pubmed
Tsai,
Molecular mechanisms of action of steroid/thyroid receptor superfamily members.
1994,
Pubmed
Voegel,
TIF2, a 160 kDa transcriptional mediator for the ligand-dependent activation function AF-2 of nuclear receptors.
1996,
Pubmed
Westin,
Interactions controlling the assembly of nuclear-receptor heterodimers and co-activators.
1998,
Pubmed
Wolffe,
Transcriptional control. Sinful repression.
1997,
Pubmed
Wolffe,
Activators and repressors: making use of chromatin to regulate transcription.
1997,
Pubmed
Wong,
Distinct requirements for chromatin assembly in transcriptional repression by thyroid hormone receptor and histone deacetylase.
1998,
Pubmed
,
Xenbase
Wong,
A role for nucleosome assembly in both silencing and activation of the Xenopus TR beta A gene by the thyroid hormone receptor.
1995,
Pubmed
,
Xenbase
Wong,
Transcription from the thyroid hormone-dependent promoter of the Xenopus laevis thyroid hormone receptor betaA gene requires a novel upstream element and the initiator, but not a TATA Box.
1998,
Pubmed
,
Xenbase
Wong,
Structural and functional features of a specific nucleosome containing a recognition element for the thyroid hormone receptor.
1997,
Pubmed
,
Xenbase
Wong,
Determinants of chromatin disruption and transcriptional regulation instigated by the thyroid hormone receptor: hormone-regulated chromatin disruption is not sufficient for transcriptional activation.
1997,
Pubmed
,
Xenbase
Workman,
Alteration of nucleosome structure as a mechanism of transcriptional regulation.
1998,
Pubmed
Xu,
Partial hormone resistance in mice with disruption of the steroid receptor coactivator-1 (SRC-1) gene.
1998,
Pubmed
Yang,
A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A.
1996,
Pubmed
Yao,
Gene dosage-dependent embryonic development and proliferation defects in mice lacking the transcriptional integrator p300.
1998,
Pubmed
Yu,
RXR beta: a coregulator that enhances binding of retinoic acid, thyroid hormone, and vitamin D receptors to their cognate response elements.
1991,
Pubmed
Zhang,
Retinoid X receptor is an auxiliary protein for thyroid hormone and retinoic acid receptors.
1992,
Pubmed
