Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proinflammatory stimuli regulate endothelial hyaluronan expression and CD44/HA-dependent primary adhesion.
Mohamadzadeh M
,
DeGrendele H
,
Arizpe H
,
Estess P
,
Siegelman M
.
???displayArticle.abstract???
The localization of circulating leukocytes within inflamed tissues occurs as the result of interactions with and migration across vascular endothelium, and is governed, in part, by the expression of adhesion molecules on both cell types. Recently, we have described a novel primary adhesion interaction between the structurally activated form of the adhesion molecule CD44 on lymphocytes and its major ligand hyaluronan on endothelial cells under physiologic laminar flow conditions, and have proposed that this interaction functions in an extravasation pathway for lymphocytes in vascular beds at sites of inflammation. While the regulation of activated CD44 on leukocytes has been characterized in depth, regulation of hyaluronate (HA) on endothelial cells has not been extensively studied. Here we demonstrate that the expression of HA on cultured endothelial cell lines and primary endothelial cultures is inducible by the proinflammatory cytokines TNFalpha and IL-1beta, as well as bacterial lipopolysaccharide. In addition, this inducibility appears strikingly restricted to endothelial cells derived from microvascular, but not large vessel, sources. The elevated HA levels thus induced result in increased CD44-dependent adhesive interactions in both nonstatic shear and laminar flow adhesion assays. Changes in mRNA levels for the described HA synthetic and degradative enzymes were not found, suggesting other more complex mechanisms of regulation. Together, these data add to the selectin and immunoglobulin gene families a new inducible endothelial adhesive molecule, hyaluronan, and help to further our understanding of the potential physiologic roles of the CD44/HA interaction; i.e., local cytokine production within inflamed vascular beds may enhance surface hyaluronan expression on endothelial cells, thereby creating local sites receptive to the CD44/HA interaction and thus extravasation of inflammatory cells.
Abbassi,
Canine neutrophil margination mediated by lectin adhesion molecule-1 in vitro.
1991, Pubmed
Abbassi,
Canine neutrophil margination mediated by lectin adhesion molecule-1 in vitro.
1991,
Pubmed
Abbassi,
E-selectin supports neutrophil rolling in vitro under conditions of flow.
1993,
Pubmed
Abbot,
Isolation and culture of synovial microvascular endothelial cells. Characterization and assessment of adhesion molecule expression.
1992,
Pubmed
Ager,
Isolation and culture of high endothelial cells from rat lymph nodes.
1987,
Pubmed
Alon,
The integrin VLA-4 supports tethering and rolling in flow on VCAM-1.
1995,
Pubmed
Alon,
Distinct cell surface ligands mediate T lymphocyte attachment and rolling on P and E selectin under physiological flow.
1994,
Pubmed
Aruffo,
CD44 is the principal cell surface receptor for hyaluronate.
1990,
Pubmed
Ausprunk,
Distribution of hyaluronic acid and sulfated glycosaminoglycans during blood-vessel development in the chick chorioallantoic membrane.
1986,
Pubmed
Bartolazzi,
Interaction between CD44 and hyaluronate is directly implicated in the regulation of tumor development.
1994,
Pubmed
Bevilacqua,
Endothelial-leukocyte adhesion molecules.
1993,
Pubmed
Bevilacqua,
Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins.
1989,
Pubmed
Bourguignon,
Hyaluronic acid-induced lymphocyte signal transduction and HA receptor (GP85/CD44)-cytoskeleton interaction.
1993,
Pubmed
Brown,
Turnover of hyaluronan in synovial joints: elimination of labelled hyaluronan from the knee joint of the rabbit.
1991,
Pubmed
Butcher,
Lymphocyte homing and homeostasis.
1996,
Pubmed
Butcher,
Leukocyte-endothelial cell recognition: three (or more) steps to specificity and diversity.
1991,
Pubmed
Camp,
CD44 is necessary for optimal contact allergic responses but is not required for normal leukocyte extravasation.
1993,
Pubmed
Campbell,
Increased hyaluronic acid is associated with dermal delayed-type hypersensitivity.
1982,
Pubmed
Carley,
Cytokeratin expression and hyaluronic acid production in cultures of human synovial microvascular endothelial cells: influence of cytokines and growth factors.
1996,
Pubmed
Carlos,
Leukocyte-endothelial adhesion molecules.
1994,
Pubmed
Clark,
CD44 and hyaluronan-dependent rolling interactions of lymphocytes on tonsillar stroma.
1996,
Pubmed
DeGrendele,
Requirement for CD44 in activated T cell extravasation into an inflammatory site.
1997,
Pubmed
DeGrendele,
CD44 and its ligand hyaluronate mediate rolling under physiologic flow: a novel lymphocyte-endothelial cell primary adhesion pathway.
1996,
Pubmed
DeGrendele,
CD44 activation and associated primary adhesion is inducible via T cell receptor stimulation.
1997,
Pubmed
Engström-Laurent,
Circulating hyaluronic acid levels vary with physical activity in healthy subjects and in rheumatoid arthritis patients. Relationship to synovitis mass and morning stiffness.
1987,
Pubmed
Engström-Laurent,
Changes in hyaluronan concentration in tissues and body fluids in disease states.
1989,
Pubmed
Engström-Laurent,
Raised serum hyaluronate levels in scleroderma: an effect of growth factor induced activation of connective tissue cells?
1985,
Pubmed
Fraser,
The kinetics of hyaluronan in normal and acutely inflamed synovial joints: observations with experimental arthritis in sheep.
1993,
Pubmed
Gerritsen,
Regulation of the expression of intercellular adhesion molecule 1 in cultured human endothelial cells derived from rheumatoid synovium.
1993,
Pubmed
Green,
Distribution of hyaluronate and hyaluronate receptors in the adult lung.
1988,
Pubmed
Hall,
Hyaluronan and the hyaluronan receptor RHAMM promote focal adhesion turnover and transient tyrosine kinase activity.
1994,
Pubmed
Haraldsen,
Cytokine-regulated expression of E-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) in human microvascular endothelial cells.
1996,
Pubmed
Harder,
Dissection of murine lymphocyte-endothelial cell interaction mechanisms by SV-40-transformed mouse endothelial cell lines: novel mechanisms mediating basal binding, and alpha 4-integrin-dependent cytokine-induced adhesion.
1991,
Pubmed
Haynes,
Measurement of an adhesion molecule as an indicator of inflammatory disease activity. Up-regulation of the receptor for hyaluronate (CD44) in rheumatoid arthritis.
1991,
Pubmed
Horibata,
Mouse myelomas and lymphomas in culture.
1970,
Pubmed
Jones,
A two-step adhesion cascade for T cell/endothelial cell interactions under flow conditions.
1994,
Pubmed
Jones,
P-selectin mediates neutrophil rolling on histamine-stimulated endothelial cells.
1993,
Pubmed
Kansas,
Selectins and their ligands: current concepts and controversies.
1996,
Pubmed
Katoh,
Glycosylation of CD44 negatively regulates its recognition of hyaluronan.
1995,
Pubmed
King,
Beneficial actions of exogenous hyaluronic acid on wound healing.
1991,
Pubmed
Laurent,
The properties and turnover of hyaluronan.
1986,
Pubmed
Lawrence,
Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins.
1991,
Pubmed
Lawrence,
Neutrophils roll on E-selectin.
1993,
Pubmed
Lesley,
Hyaluronan binding function of CD44 is transiently activated on T cells during an in vivo immune response.
1994,
Pubmed
Lesley,
Requirements for hyaluronic acid binding by CD44: a role for the cytoplasmic domain and activation by antibody.
1992,
Pubmed
Lesley,
CD44 and its interaction with extracellular matrix.
1993,
Pubmed
Lesley,
Variant cell lines selected for alterations in the function of the hyaluronan receptor CD44 show differences in glycosylation.
1995,
Pubmed
Lundin,
Circulating hyaluronate in psoriasis.
1985,
Pubmed
Lundin,
High levels of hyaluronate in suction blister fluid from active psoriatic lesions.
1987,
Pubmed
Mantovani,
Cytokine regulation of endothelial cell function.
1992,
Pubmed
McHeyzer-Williams,
Antigen-specific development of primary and memory T cells in vivo.
1995,
Pubmed
Mikecz,
Anti-CD44 treatment abrogates tissue oedema and leukocyte infiltration in murine arthritis.
1995,
Pubmed
Miyake,
Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition.
1990,
Pubmed
Miyake,
Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures.
1990,
Pubmed
Oksala,
Expression of proteoglycans and hyaluronan during wound healing.
1995,
Pubmed
Pitsillides,
Hyaluronan concentration in non-inflamed and rheumatoid synovium.
1994,
Pubmed
Pober,
Cytokines and endothelial cell biology.
1990,
Pubmed
Rice,
An inducible endothelial cell surface glycoprotein mediates melanoma adhesion.
1989,
Pubmed
Saiki,
Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes.
,
Pubmed
Spertini,
Leukocyte adhesion molecule-1 (LAM-1, L-selectin) interacts with an inducible endothelial cell ligand to support leukocyte adhesion.
1991,
Pubmed
Springer,
Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm.
1994,
Pubmed
Stamenkovic,
The hematopoietic and epithelial forms of CD44 are distinct polypeptides with different adhesion potentials for hyaluronate-bearing cells.
1991,
Pubmed
Swerlick,
Studies of the modulation of MHC antigen and cell adhesion molecule expression on human dermal microvascular endothelial cells.
1991,
Pubmed
Tammi,
Hyaluronan and CD44 in psoriatic skin. Intense staining for hyaluronan on dermal capillary loops and reduced expression of CD44 and hyaluronan in keratinocyte-leukocyte interfaces.
1994,
Pubmed
Thornhill,
IL-4 increases human endothelial cell adhesiveness for T cells but not for neutrophils.
1990,
Pubmed
